Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5066181..5066678 Replicon chromosome
Accession NZ_CP089272
Organism Escherichia coli O157:H7 strain M1300706001A

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LSI73_RS25525 Protein ID WP_001173343.1
Coordinates 5066181..5066354 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LSI73_RS25530 Protein ID WP_000398619.1
Coordinates 5066382..5066678 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSI73_RS25515 (5062851) 5062851..5064308 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
LSI73_RS25520 (5064545) 5064545..5066062 + 1518 WP_001295074.1 lysine--tRNA ligase -
LSI73_RS25525 (5066181) 5066181..5066354 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LSI73_RS25530 (5066382) 5066382..5066678 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LSI73_RS25535 (5066905) 5066905..5067177 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LSI73_RS25540 (5067189) 5067189..5067419 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
LSI73_RS25545 (5067600) 5067600..5069231 + 1632 WP_001216477.1 sensor histidine kinase -
LSI73_RS25550 (5069228) 5069228..5069947 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T227972 WP_001173343.1 NZ_CP089272:c5066354-5066181 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T227972 NZ_CP123244:204815-204922 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT227972 WP_000398619.1 NZ_CP089272:c5066678-5066382 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT227972 NZ_CP123244:c204766-204703 [Escherichia coli]
GTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References