Detailed information of TA system TA227971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4969207..4969802
• Replicon: chromosome
• Accession: NZ_CP089272
• Organism: Escherichia coli O157:H7 strain M1300706001A

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: LSI73_RS25025
• Protein ID: WP_000239579.1
• Coordinates: 4969207..4969557 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: LSI73_RS25030
• Protein ID: WP_001223210.1
• Coordinates: 4969551..4969802 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSI73_RS25005 (4964653)	4964653..4965675	-	1023	WP_001301928.1	ABC transporter permease	-
LSI73_RS25010 (4965689)	4965689..4967191	-	1503	Protein_4903	sugar ABC transporter ATP-binding protein	-
LSI73_RS25015 (4967331)	4967331..4968287	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
LSI73_RS25020 (4968597)	4968597..4969127	+	531	WP_000055075.1	inorganic diphosphatase	-
LSI73_RS25025 (4969207)	4969207..4969557	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
LSI73_RS25030 (4969551)	4969551..4969802	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
LSI73_RS25035 (4970014)	4970014..4970355	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
LSI73_RS25040 (4970358)	4970358..4974137	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T227971 WP_000239579.1 NZ_CP089272:c4969557-4969207 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T227971 NZ_CP123241:106268-106666 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT227971 WP_001223210.1 NZ_CP089272:c4969802-4969551 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT227971 NZ_CP123241:106041-106268 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8