Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4181127..4181745 Replicon chromosome
Accession NZ_CP089272
Organism Escherichia coli O157:H7 strain M1300706001A

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LSI73_RS21380 Protein ID WP_001291435.1
Coordinates 4181527..4181745 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LSI73_RS21375 Protein ID WP_000344800.1
Coordinates 4181127..4181501 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSI73_RS21365 (4176216) 4176216..4177409 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LSI73_RS21370 (4177432) 4177432..4180581 + 3150 WP_001132452.1 efflux RND transporter permease AcrB -
LSI73_RS21375 (4181127) 4181127..4181501 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LSI73_RS21380 (4181527) 4181527..4181745 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LSI73_RS21385 (4181917) 4181917..4182468 + 552 WP_000102553.1 maltose O-acetyltransferase -
LSI73_RS21390 (4182584) 4182584..4183054 + 471 WP_000136192.1 YlaC family protein -
LSI73_RS21395 (4183218) 4183218..4184768 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LSI73_RS21400 (4184810) 4184810..4185163 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LSI73_RS21410 (4185542) 4185542..4185853 + 312 WP_000409911.1 MGMT family protein -
LSI73_RS21415 (4185886) 4185886..4186458 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T227966 WP_001291435.1 NZ_CP089272:4181527-4181745 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T227966 NZ_CP123240:c4551113-4550763 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT227966 WP_000344800.1 NZ_CP089272:4181127-4181501 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT227966 NZ_CP123240:c4551358-4551107 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References