Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2625156..2625627 Replicon chromosome
Accession NZ_CP089272
Organism Escherichia coli O157:H7 strain M1300706001A

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag LSI73_RS13410 Protein ID WP_001303511.1
Coordinates 2625156..2625434 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag LSI73_RS13415 Protein ID WP_001302048.1
Coordinates 2625436..2625627 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSI73_RS13385 (2622091) 2622091..2623176 - 1086 Protein_2619 exonuclease -
LSI73_RS13390 (2623269) 2623269..2623460 - 192 WP_001090200.1 DUF1482 family protein -
LSI73_RS13395 (2623457) 2623457..2623645 - 189 WP_000449192.1 cell division inhibition protein DicB -
LSI73_RS13400 (2624214) 2624214..2624432 - 219 WP_001171930.1 protein YdfC -
LSI73_RS13405 (2624504) 2624504..2624803 - 300 WP_001240334.1 hypothetical protein -
LSI73_RS13410 (2625156) 2625156..2625434 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LSI73_RS13415 (2625436) 2625436..2625627 - 192 WP_001302048.1 hypothetical protein Antitoxin
LSI73_RS13420 (2625648) 2625648..2626019 - 372 WP_001169686.1 hypothetical protein -
LSI73_RS13425 (2626117) 2626117..2626419 + 303 WP_000172738.1 transcriptional regulator -
LSI73_RS13430 (2626416) 2626416..2626841 + 426 WP_000693943.1 toxin YdaT family protein -
LSI73_RS13435 (2626864) 2626864..2627826 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
LSI73_RS13440 (2627833) 2627833..2628573 + 741 WP_000788938.1 ATP-binding protein -
LSI73_RS13445 (2628599) 2628599..2629368 + 770 Protein_2631 DUF1627 domain-containing protein -
LSI73_RS13450 (2629384) 2629384..2629779 + 396 WP_001118159.1 DUF977 family protein -
LSI73_RS13455 (2629836) 2629836..2630420 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 2608963..2709817 100854


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T227957 WP_001303511.1 NZ_CP089272:c2625434-2625156 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T227957 NZ_CP123240:2913991-2914098 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT227957 WP_001302048.1 NZ_CP089272:c2625627-2625436 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT227957 NZ_CP123240:c2913943-2913876 [Escherichia coli]
GTCTAGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References