Detailed information of TA system TA022795

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ParST/RES-Xre
• Location: 243389..244368
• Replicon: plasmid pBMC401
• Accession: NC_010553
• Organism: Burkholderia ambifaria MC40-6

Toxin (Protein)

• Gene name: ParT
• Uniprot ID: B1Z6T2
• Locus tag: BAMMC406_RS33615
• Protein ID: WP_012367391.1
• Coordinates: 243889..244368 (+)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: ParS
• Uniprot ID: B1Z6T1
• Locus tag: BAMMC406_RS33610
• Protein ID: WP_012367390.1
• Coordinates: 243389..243892 (+)
• Length: 168 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAMMC406_RS33595	239574..241475	-	1902	WP_012367388.1	hypothetical protein	-
BAMMC406_RS33600	241490..242668	-	1179	WP_012367389.1	site-specific integrase	-
BAMMC406_RS33610	243389..243892	+	504	WP_012367390.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
BAMMC406_RS33615	243889..244368	+	480	WP_012367391.1	RES family NAD+ phosphorylase	Toxin
BAMMC406_RS33620	244381..245391	-	1011	WP_050772617.1	hypothetical protein	-
BAMMC406_RS33625	245415..245804	-	390	WP_175775248.1	hypothetical protein	-
BAMMC406_RS33630	245886..246734	-	849	WP_012367394.1	peptidyl-prolyl cis-trans isomerase	-
BAMMC406_RS33635	246735..248477	-	1743	WP_050772619.1	ShlB/FhaC/HecB family hemolysin secretion/activation protein	-
BAMMC406_RS35300	248528..248686	-	159	WP_175775239.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hsiB1/vipA / hsiC1/vipB	1..301592	301592

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (5-151)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (40-99)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 17205.50 Da
• Isoelectric Point: 4.8996

>T22795 WP_012367391.1 NC_010553:243889-244368 [Burkholderia ambifaria MC40-6]
MTVALWRIATDTPAYTADDMSGEGAKRTGGRWNRQDRAVLYTASNVALACLETVVHLSGGDLPLNRYLVRFDVPDEVWAK
ASTLDAASAPVGWDALPAGKVSLDLGDAWLSEGATALMRVPSVIVPEETNVLINPAHADAAQITVTKLRRWTYDARVWA

Nucleotide

• Length: 480 bp

>T22795 NC_010553:243889-244368 [Burkholderia ambifaria MC40-6]
ATGACGGTCGCGCTCTGGCGAATCGCCACCGATACGCCGGCCTACACGGCAGACGATATGAGCGGCGAAGGGGCGAAGCG
AACAGGCGGCAGGTGGAACCGCCAAGACCGCGCTGTGTTGTACACGGCCTCGAACGTCGCCCTCGCCTGCCTCGAGACAG
TCGTGCATCTGAGCGGCGGGGACCTTCCCCTGAACCGCTATCTGGTCCGATTCGACGTCCCCGATGAGGTATGGGCGAAA
GCGAGCACCCTCGACGCGGCGTCGGCGCCGGTGGGCTGGGATGCGTTGCCGGCCGGTAAGGTCAGCCTCGATCTCGGCGA
TGCCTGGCTGTCTGAAGGCGCCACCGCGCTTATGCGCGTGCCATCCGTCATCGTCCCGGAAGAGACGAACGTGCTGATCA
ATCCGGCGCACGCCGATGCCGCACAGATCACCGTCACCAAACTGCGGCGCTGGACCTATGACGCCCGCGTATGGGCGTAG

Antitoxin

Protein

• Length: 168 a.a.
• Molecular weight: 18477.15 Da
• Isoelectric Point: 6.8850

>AT22795 WP_012367390.1 NC_010553:243389-243892 [Burkholderia ambifaria MC40-6]
MSAIEKRHTEPARARPLAAEAHEFEVLDFQRTYRASPMERIDWIKHGVDAHIVILLADRMATTQDWLIGILGFPRTTVVR
KSKAKQPLSTEQTERVVGLSKLIGQVQSMVEESGEPAGFDAAQWVAQWIERANPALGGRKPAELMDTVAGQDLVSTVLAR
MQSGAYA

Nucleotide

• Length: 504 bp

>AT22795 NC_010553:243389-243892 [Burkholderia ambifaria MC40-6]
ATGAGCGCCATCGAGAAACGACACACCGAGCCGGCACGAGCACGACCGCTCGCAGCTGAAGCGCACGAGTTCGAGGTCCT
AGACTTTCAGCGGACCTATCGCGCATCGCCGATGGAACGCATCGACTGGATCAAACACGGGGTCGACGCCCACATCGTGA
TCCTGCTCGCCGATCGCATGGCGACCACGCAGGATTGGCTCATAGGTATCCTCGGATTTCCGCGCACGACAGTGGTTCGG
AAGTCGAAGGCCAAGCAACCGCTGTCGACCGAACAGACCGAACGCGTCGTCGGACTATCGAAGCTGATCGGTCAGGTTCA
GTCGATGGTGGAGGAATCCGGCGAGCCGGCAGGTTTCGATGCCGCGCAGTGGGTCGCGCAGTGGATCGAGCGAGCCAACC
CCGCCTTGGGCGGCCGCAAGCCCGCCGAACTGATGGATACCGTCGCTGGTCAGGATCTCGTCAGCACCGTGCTCGCACGA
ATGCAAAGCGGAGCGTATGCATGA

Structures

Toxin

• AlphaFold DB: B1Z6T2

Antitoxin

• AlphaFold DB: B1Z6T1