Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 940252..940906 Replicon chromosome
Accession NZ_CP089272
Organism Escherichia coli O157:H7 strain M1300706001A

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LSI73_RS04650 Protein ID WP_000244781.1
Coordinates 940499..940906 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LSI73_RS04645 Protein ID WP_000354046.1
Coordinates 940252..940518 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSI73_RS04625 (936340) 936340..937773 - 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -
LSI73_RS04630 (937818) 937818..938129 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
LSI73_RS04635 (938293) 938293..938952 + 660 WP_000250274.1 hemolysin III family protein -
LSI73_RS04640 (939029) 939029..940009 - 981 WP_000886053.1 tRNA-modifying protein YgfZ -
LSI73_RS04645 (940252) 940252..940518 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LSI73_RS04650 (940499) 940499..940906 + 408 WP_000244781.1 protein YgfX Toxin
LSI73_RS04655 (940946) 940946..941467 - 522 WP_001055874.1 flavodoxin FldB -
LSI73_RS04660 (941579) 941579..942475 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LSI73_RS04665 (942500) 942500..943210 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LSI73_RS04670 (943216) 943216..944949 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T227947 WP_000244781.1 NZ_CP089272:940499-940906 [Escherichia coli O157:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T227947 NZ_CP123240:232244-232351 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT227947 WP_000354046.1 NZ_CP089272:940252-940518 [Escherichia coli O157:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT227947 NZ_CP123240:c232195-232132 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References