Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 359393..360024 Replicon chromosome
Accession NZ_CP089272
Organism Escherichia coli O157:H7 strain M1300706001A

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag LSI73_RS01675 Protein ID WP_001259385.1
Coordinates 359393..359668 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag LSI73_RS01680 Protein ID WP_000593555.1
Coordinates 359665..360024 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSI73_RS01660 (354397) 354397..355464 + 1068 WP_000361458.1 HlyD family secretion protein -
LSI73_RS01665 (355461) 355461..358196 + 2736 WP_000149110.1 ribosome-associated ATPase/putative transporter RbbA -
LSI73_RS01670 (358196) 358196..359320 + 1125 WP_001301659.1 ABC transporter permease -
LSI73_RS01675 (359393) 359393..359668 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
LSI73_RS01680 (359665) 359665..360024 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LSI73_RS01685 (360074) 360074..360934 - 861 WP_001131788.1 class II aldolase -
LSI73_RS01690 (360966) 360966..361235 - 270 WP_000933060.1 HPr family phosphocarrier protein -
LSI73_RS01695 (361225) 361225..362733 - 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
LSI73_RS01700 (362726) 362726..364084 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
LSI73_RS01705 (364161) 364161..364442 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
LSI73_RS01710 (364439) 364439..364912 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 350537..360024 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T227942 WP_001259385.1 NZ_CP089272:359393-359668 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T227942 NZ_CP123237:c4259880-4259707 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT227942 WP_000593555.1 NZ_CP089272:359665-360024 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT227942 NZ_CP123237:c4260204-4259908 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References