Detailed information of TA system TA022793

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 229122..229684
• Replicon: plasmid pBMC401
• Accession: NC_010553
• Organism: Burkholderia ambifaria MC40-6

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: B1Z6R5
• Locus tag: BAMMC406_RS33530
• Protein ID: WP_012367374.1
• Coordinates: 229122..229442 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BAMMC406_RS33535
• Protein ID: WP_041490363.1
• Coordinates: 229436..229684 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAMMC406_RS33500	224236..224565	-	330	WP_012367367.1	hypothetical protein	-
BAMMC406_RS33505	224593..225567	-	975	WP_012367368.1	hypothetical protein	-
BAMMC406_RS33510	225581..226093	-	513	WP_012367369.1	hypothetical protein	-
BAMMC406_RS33515	226112..226930	-	819	WP_041490362.1	abortive infection family protein	-
BAMMC406_RS33520	226948..227394	-	447	WP_012367371.1	hypothetical protein	-
BAMMC406_RS35275	227413..227631	-	219	WP_175712422.1	hypothetical protein	-
BAMMC406_RS35280	227957..228229	-	273	WP_175775300.1	hypothetical protein	-
BAMMC406_RS35285	228245..228511	-	267	WP_012367357.1	hypothetical protein	-
BAMMC406_RS33525	228527..228913	-	387	WP_050772613.1	hypothetical protein	-
BAMMC406_RS35290	228932..229099	-	168	WP_012367373.1	hypothetical protein	-
BAMMC406_RS33530	229122..229442	-	321	WP_012367374.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BAMMC406_RS33535	229436..229684	-	249	WP_041490363.1	antitoxin	Antitoxin
BAMMC406_RS33540	229772..230461	-	690	WP_041490364.1	site-specific integrase	-
BAMMC406_RS33545	230634..230960	+	327	WP_012367377.1	H-NS histone family protein	-
BAMMC406_RS33550	231088..231879	+	792	WP_012367378.1	SOS response-associated peptidase family protein	-
BAMMC406_RS35295	232122..232448	-	327	WP_012367379.1	hypothetical protein	-
BAMMC406_RS33555	232641..233528	-	888	WP_012367380.1	ImmA/IrrE family metallo-endopeptidase	-
BAMMC406_RS33560	233532..233879	-	348	WP_012367381.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hsiB1/vipA / hsiC1/vipB	1..301592	301592

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-104)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11876.13 Da
• Isoelectric Point: 10.5567

>T22793 WP_012367374.1 NC_010553:c229442-229122 [Burkholderia ambifaria MC40-6]
MVVRGEVWLVALDPTMGSEIQKTRPCVVVSPPEMHDHLRTVLVAPMTSKGRAAPFRIPVTFKRKQGLILLDQMRAVDKVR
LVKKEGAVADKTLLDTLRTLQEVFAE

Nucleotide

• Length: 321 bp

>T22793 NC_010553:c229442-229122 [Burkholderia ambifaria MC40-6]
ATGGTAGTGCGCGGAGAAGTTTGGTTGGTCGCGCTCGATCCGACGATGGGCAGCGAAATCCAGAAAACGCGCCCTTGCGT
CGTGGTTTCGCCTCCCGAAATGCACGATCATCTGCGGACAGTTCTCGTCGCGCCGATGACTTCGAAGGGCCGCGCCGCAC
CTTTTCGGATTCCGGTGACGTTCAAGCGAAAGCAGGGCTTAATCCTTCTGGATCAGATGCGTGCGGTAGACAAGGTGCGA
CTGGTCAAGAAAGAAGGCGCAGTGGCCGATAAAACGTTGTTGGATACCCTGCGCACGTTGCAGGAAGTCTTCGCGGAATA
G

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9034.23 Da
• Isoelectric Point: 4.4929

>AT22793 WP_041490363.1 NC_010553:c229684-229436 [Burkholderia ambifaria MC40-6]
MKTTIRRMGNSQGVLIPKPVLAQLGLENEVEMEVENDAIVLRRPRHKAREGWAEASKALAESGDDALVMGEFSNADDAEL
AW

Nucleotide

• Length: 249 bp

>AT22793 NC_010553:c229684-229436 [Burkholderia ambifaria MC40-6]
ATGAAAACGACGATTCGCAGGATGGGTAACTCGCAAGGCGTGCTGATTCCCAAGCCCGTCCTCGCGCAGTTGGGATTGGA
GAACGAAGTGGAAATGGAAGTCGAAAACGACGCGATTGTGCTGCGCCGCCCCCGGCACAAGGCCCGTGAAGGATGGGCGG
AAGCGAGCAAGGCGCTCGCGGAGAGTGGTGATGATGCGCTTGTGATGGGTGAATTCTCCAATGCCGACGATGCGGAGCTT
GCATGGTAG

Structures

Toxin

• AlphaFold DB: B1Z6R5