Detailed information of TA system TA227927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2763775..2764413
• Replicon: chromosome
• Accession: NZ_CP089270
• Organism: Escherichia coli O157:H7 strain M1300706002

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: LSI72_RS13975
• Protein ID: WP_000813794.1
• Coordinates: 2764237..2764413 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LSI72_RS13970
• Protein ID: WP_001270286.1
• Coordinates: 2763775..2764191 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSI72_RS13950 (2758927)	2758927..2759868	-	942	WP_001251320.1	ABC transporter permease	-
LSI72_RS13955 (2759869)	2759869..2760882	-	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
LSI72_RS13960 (2760900)	2760900..2762045	-	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
LSI72_RS13965 (2762290)	2762290..2763696	-	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
LSI72_RS13970 (2763775)	2763775..2764191	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LSI72_RS13975 (2764237)	2764237..2764413	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LSI72_RS13980 (2764635)	2764635..2764865	+	231	WP_000494244.1	YncJ family protein	-
LSI72_RS13985 (2764957)	2764957..2766918	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LSI72_RS13990 (2766991)	2766991..2767527	-	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
LSI72_RS13995 (2767580)	2767580..2768791	+	1212	WP_071525382.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T227927 WP_000813794.1 NZ_CP089270:c2764413-2764237 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T227927 NZ_CP123237:821256-821663 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT227927 WP_001270286.1 NZ_CP089270:c2764191-2763775 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT227927 NZ_CP123237:821009-821275 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 6HPB