Detailed information of TA system TA022792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 71555..72229
• Replicon: plasmid pBMC401
• Accession: NC_010553
• Organism: Burkholderia ambifaria MC40-6

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BAMMC406_RS35100
• Protein ID: WP_012367237.1
• Coordinates: 71555..71749 (+)
• Length: 65 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B1Z6C7
• Locus tag: BAMMC406_RS32825
• Protein ID: WP_012367238.1
• Coordinates: 71816..72229 (+)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAMMC406_RS32810	66894..67868	+	975	WP_012367235.1	ParB/RepB/Spo0J family partition protein	-
BAMMC406_RS32815	68348..70480	-	2133	WP_012367236.1	tyrosine-type recombinase/integrase	-
BAMMC406_RS35095	70918..71079	-	162	WP_175775267.1	hypothetical protein	-
BAMMC406_RS35100	71555..71749	+	195	WP_012367237.1	type II toxin-antitoxin system HicA family toxin	Toxin
BAMMC406_RS32825	71816..72229	+	414	WP_012367238.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BAMMC406_RS35105	72605..72871	+	267	Protein_76	transposase	-
BAMMC406_RS32830	72930..73955	+	1026	WP_012367239.1	IS110 family transposase	-
BAMMC406_RS32835	74208..75150	+	943	Protein_78	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hsiB1/vipA / hsiC1/vipB	1..301592	301592

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7280.30 Da
• Isoelectric Point: 11.2466

>T22792 WP_012367237.1 NC_010553:71555-71749 [Burkholderia ambifaria MC40-6]
MDSRTVIRRLEQDGWVQKRVKGSHHHFRHPSKPGTVTVPHPEKDLTIATLKSIERQSGLTLTGQ

Nucleotide

• Length: 195 bp

>T22792 NC_010553:71555-71749 [Burkholderia ambifaria MC40-6]
ATGGATTCGAGAACAGTGATACGGCGGCTTGAGCAGGATGGGTGGGTGCAGAAGCGGGTGAAGGGTAGTCATCATCACTT
CAGGCACCCGAGCAAGCCAGGCACGGTAACGGTGCCGCATCCAGAGAAAGACCTCACGATTGCGACGCTGAAAAGCATCG
AGAGGCAATCTGGTTTAACACTCACCGGGCAGTAA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15384.04 Da
• Isoelectric Point: 4.1066

>AT22792 WP_012367238.1 NC_010553:71816-72229 [Burkholderia ambifaria MC40-6]
VRFFVAVDKQEGTDYGVVVPDFPGCNSWGTTLEQALENTQEAIEGWVETSVEAGDEVRFEPTSFEELRDNPDYAGSFWAV
VDIDPSKFDMKPERVNISLPRFVLTQIDAFAEAHKETRSGFLVRAALEAIRHEQTAV

Nucleotide

• Length: 414 bp

>AT22792 NC_010553:71816-72229 [Burkholderia ambifaria MC40-6]
GTGAGATTTTTCGTCGCTGTCGACAAGCAAGAGGGCACCGATTATGGCGTTGTCGTGCCGGATTTTCCGGGCTGCAACTC
ATGGGGCACGACGCTCGAACAAGCGCTCGAAAACACGCAAGAGGCTATCGAGGGATGGGTTGAAACTTCCGTCGAGGCGG
GGGACGAAGTTCGCTTCGAGCCCACGTCGTTTGAGGAACTTCGCGACAACCCAGACTATGCTGGCTCATTTTGGGCTGTC
GTAGACATTGATCCTTCGAAATTTGATATGAAGCCAGAGCGTGTGAATATAAGTTTGCCGCGCTTCGTGCTTACACAGAT
TGACGCCTTCGCGGAGGCCCACAAGGAAACGCGTAGCGGCTTTCTTGTTCGTGCGGCACTAGAAGCCATTCGGCACGAGC
AGACAGCCGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3N8L2T7