Detailed information of TA system TA227909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4406303..4407561
• Replicon: chromosome
• Accession: NZ_CP089238
• Organism: Pseudomonas aeruginosa strain JNQH-PA033

Toxin (Protein)

• Gene name: -
• Uniprot ID: V6AJ34
• Locus tag: LSG45_RS20320
• Protein ID: WP_003086331.1
• Coordinates: 4406303..4407058 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: LSG45_RS20325
• Protein ID: WP_003086329.1
• Coordinates: 4407193..4407561 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSG45_RS20310 (LSG45_20310)	4401474..4404017	+	2544	WP_083567487.1	acyl-homoserine-lactone acylase QuiP	-
LSG45_RS20315 (LSG45_20315)	4404172..4405533	-	1362	WP_234575781.1	DNA recombination protein RmuC	-
LSG45_RS20320 (LSG45_20320)	4406303..4407058	-	756	WP_003086331.1	RES family NAD+ phosphorylase	Toxin
LSG45_RS20325 (LSG45_20325)	4407193..4407561	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
LSG45_RS20330 (LSG45_20330)	4407634..4408920	-	1287	WP_234574780.1	FAD-binding oxidoreductase	-
LSG45_RS20335 (LSG45_20335)	4408976..4410565	-	1590	WP_079980225.1	aldehyde dehydrogenase family protein	-
LSG45_RS20340 (LSG45_20340)	4410643..4411119	+	477	WP_009877993.1	elongation factor GreAB	-
LSG45_RS20345 (LSG45_20345)	4411122..4411397	-	276	WP_023088486.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28236.34 Da
• Isoelectric Point: 7.7294

>T227909 WP_003086331.1 NZ_CP089238:c4407058-4406303 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T227909 NZ_CP123231:c3507881-3507579 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT227909 WP_003086329.1 NZ_CP089238:c4407561-4407193 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT227909 NZ_CP123231:c3507543-3507202 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: V6AJ34

Antitoxin

• AlphaFold DB: V6AJU7