Detailed information of TA system TA227903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 26014..26564
• Replicon: plasmid pPA027
• Accession: NZ_CP089237
• Organism: Pseudomonas aeruginosa strain JNQH-PA027

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6B1YJ09
• Locus tag: LSG40_RS33785
• Protein ID: WP_034019113.1
• Coordinates: 26289..26564 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A6B1YL09
• Locus tag: LSG40_RS33780
• Protein ID: WP_019727126.1
• Coordinates: 26014..26289 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSG40_RS33740 (LSG40_33740)	22342..22605	-	264	WP_084929135.1	hypothetical protein	-
LSG40_RS33745 (LSG40_33745)	22619..22891	-	273	WP_084929133.1	hypothetical protein	-
LSG40_RS33750 (LSG40_33750)	22888..23139	-	252	WP_058159039.1	hypothetical protein	-
LSG40_RS33755 (LSG40_33755)	23292..23450	+	159	WP_234493642.1	hypothetical protein	-
LSG40_RS33760 (LSG40_33760)	23501..23749	-	249	WP_033992320.1	hypothetical protein	-
LSG40_RS33765 (LSG40_33765)	24368..24676	-	309	WP_058159041.1	TrfB-related DNA-binding protein	-
LSG40_RS33770 (LSG40_33770)	25000..25293	-	294	WP_033992327.1	hypothetical protein	-
LSG40_RS33775 (LSG40_33775)	25354..25740	-	387	WP_034019111.1	hypothetical protein	-
LSG40_RS33780 (LSG40_33780)	26014..26289	+	276	WP_019727126.1	ribbon-helix-helix domain-containing protein	Antitoxin
LSG40_RS33785 (LSG40_33785)	26289..26564	+	276	WP_034019113.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSG40_RS33790 (LSG40_33790)	26567..26830	+	264	WP_172405635.1	hypothetical protein	-
LSG40_RS33795 (LSG40_33795)	27702..28022	-	321	WP_034019116.1	hypothetical protein	-
LSG40_RS33800 (LSG40_33800)	28042..28464	-	423	WP_084929126.1	hypothetical protein	-
LSG40_RS33805 (LSG40_33805)	28469..29551	-	1083	WP_084929124.1	DNA-binding protein	-
LSG40_RS33810 (LSG40_33810)	29835..30629	-	795	WP_234493643.1	DUF3560 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..46776	46776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-48)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10751.35 Da
• Isoelectric Point: 7.1657

>T227903 WP_034019113.1 NZ_CP089237:26289-26564 [Pseudomonas aeruginosa]
MELKWTSKGLSDVARLYEFLATVNKEAAARTVQQLTSAPTALLTNPRLGERLEEFEPRDVRRILIGHYEMRYEILDSCIY
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T227903 NZ_CP123231:1130513-1130848 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGA
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10142.32 Da
• Isoelectric Point: 4.8433

>AT227903 WP_019727126.1 NZ_CP089237:26014-26289 [Pseudomonas aeruginosa]
MSTAAKPTRTVTAHVPVDLAERVDAIAERLERSKNWIVRQALSAWIDQEDERSRLTREALDDVDAGRVVDHQAVQAWAES
LGTDSPLPVPR

Nucleotide

• Length: 276 bp

>AT227903 NZ_CP123231:1130265-1130513 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A6B1YJ09

Antitoxin

• AlphaFold DB: A0A6B1YL09