Detailed information of TA system TA227901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5521636..5522222
• Replicon: chromosome
• Accession: NZ_CP089236
• Organism: Pseudomonas aeruginosa strain JNQH-PA027

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: LSG40_RS26750
• Protein ID: WP_003120987.1
• Coordinates: 5521923..5522222 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: LSG40_RS26745
• Protein ID: WP_003448662.1
• Coordinates: 5521636..5521926 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSG40_RS26730 (LSG40_26730)	5517212..5519107	+	1896	WP_234493604.1	hypothetical protein	-
LSG40_RS26735 (LSG40_26735)	5519104..5521080	+	1977	WP_042931628.1	DEAD/DEAH box helicase	-
LSG40_RS33970	5521090..5521224	+	135	WP_033179080.1	hypothetical protein	-
LSG40_RS26740 (LSG40_26740)	5521221..5521565	+	345	WP_003448665.1	hypothetical protein	-
LSG40_RS26745 (LSG40_26745)	5521636..5521926	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
LSG40_RS26750 (LSG40_26750)	5521923..5522222	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSG40_RS26755 (LSG40_26755)	5522424..5523545	+	1122	WP_003448658.1	TcpQ domain-containing protein	-
LSG40_RS26760 (LSG40_26760)	5523545..5525254	+	1710	WP_010792227.1	PilN family type IVB pilus formation outer membrane protein	-
LSG40_RS26765 (LSG40_26765)	5525258..5526583	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
LSG40_RS26770 (LSG40_26770)	5526573..5527106	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5490395..5577759	87364

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T227901 WP_003120987.1 NZ_CP089236:c5522222-5521923 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T227901 NZ_CP123231:767066-767362 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT227901 WP_003448662.1 NZ_CP089236:c5521926-5521636 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT227901 NZ_CP123231:767364-767759 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: G8CP73