Detailed information of TA system TA227900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4631805..4633063
• Replicon: chromosome
• Accession: NZ_CP089236
• Organism: Pseudomonas aeruginosa strain JNQH-PA027

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: LSG40_RS22165
• Protein ID: WP_003112528.1
• Coordinates: 4631805..4632560 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: LSG40_RS22170
• Protein ID: WP_003086329.1
• Coordinates: 4632695..4633063 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSG40_RS22155 (LSG40_22155)	4626978..4629521	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
LSG40_RS22160 (LSG40_22160)	4629676..4631037	-	1362	WP_010895536.1	DNA recombination protein RmuC	-
LSG40_RS22165 (LSG40_22165)	4631805..4632560	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
LSG40_RS22170 (LSG40_22170)	4632695..4633063	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
LSG40_RS22175 (LSG40_22175)	4633136..4634422	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
LSG40_RS22180 (LSG40_22180)	4634478..4636067	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
LSG40_RS22185 (LSG40_22185)	4636146..4636622	+	477	WP_003112531.1	hypothetical protein	-
LSG40_RS22190 (LSG40_22190)	4636625..4636900	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T227900 WP_003112528.1 NZ_CP089236:c4632560-4631805 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T227900 NZ_CP123231:702491-702805 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT227900 WP_003086329.1 NZ_CP089236:c4633063-4632695 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT227900 NZ_CP123231:702802-703218 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7