Detailed information of TA system TA227898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2698680..2699901
• Replicon: chromosome
• Accession: NZ_CP089236
• Organism: Pseudomonas aeruginosa strain JNQH-PA027

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: LSG40_RS13200
• Protein ID: WP_003102740.1
• Coordinates: 2698680..2699258 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LSG40_RS13205
• Protein ID: WP_003088112.1
• Coordinates: 2699347..2699901 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSG40_RS13185 (LSG40_13185)	2693956..2695233	+	1278	WP_003102743.1	TolC family protein	-
LSG40_RS13190 (LSG40_13190)	2695223..2697394	+	2172	WP_003114950.1	type I secretion system permease/ATPase	-
LSG40_RS13195 (LSG40_13195)	2697384..2698571	+	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
LSG40_RS13200 (LSG40_13200)	2698680..2699258	-	579	WP_003102740.1	HD domain-containing protein	Toxin
LSG40_RS13205 (LSG40_13205)	2699347..2699901	-	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
LSG40_RS13210 (LSG40_13210)	2700059..2702254	-	2196	WP_010794059.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
LSG40_RS13215 (LSG40_13215)	2702259..2702720	-	462	WP_003102736.1	(2Fe-2S)-binding protein	-
LSG40_RS13220 (LSG40_13220)	2703051..2703374	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
LSG40_RS13225 (LSG40_13225)	2703387..2703785	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
LSG40_RS13230 (LSG40_13230)	2703782..2704333	-	552	WP_004343115.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	tse1 / lasA	2535591..2707384	171793

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T227898 WP_003102740.1 NZ_CP089236:c2699258-2698680 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T227898 NZ_CP123231:c44219-43842 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT227898 WP_003088112.1 NZ_CP089236:c2699901-2699347 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT227898 NZ_CP123231:c44640-44266 [Escherichia coli]
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTTGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07