Detailed information of TA system TA227895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 160841..161346
• Replicon: chromosome
• Accession: NZ_CP089236
• Organism: Pseudomonas aeruginosa strain JNQH-PA027

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: LSG40_RS00740
• Protein ID: WP_003083773.1
• Coordinates: 160841..161122 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: LSG40_RS00745
• Protein ID: WP_003083775.1
• Coordinates: 161119..161346 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSG40_RS00715 (LSG40_00715)	156092..157441	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
LSG40_RS00720 (LSG40_00720)	157490..158176	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
LSG40_RS00725 (LSG40_00725)	158277..159011	+	735	WP_003117208.1	GntR family transcriptional regulator	-
LSG40_RS00730 (LSG40_00730)	159191..159601	+	411	WP_003101225.1	aegerolysin family protein	-
LSG40_RS00735 (LSG40_00735)	159633..160541	-	909	WP_010793581.1	LysR family transcriptional regulator	-
LSG40_RS00740 (LSG40_00740)	160841..161122	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
LSG40_RS00745 (LSG40_00745)	161119..161346	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
LSG40_RS00750 (LSG40_00750)	161522..162142	-	621	WP_003101226.1	hypothetical protein	-
LSG40_RS00755 (LSG40_00755)	162243..162743	+	501	WP_003101228.1	LEA type 2 family protein	-
LSG40_RS00760 (LSG40_00760)	162816..163157	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
LSG40_RS00765 (LSG40_00765)	163239..164666	-	1428	WP_003083784.1	GABA permease	-
LSG40_RS00770 (LSG40_00770)	164835..166328	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T227895 WP_003083773.1 NZ_CP089236:c161122-160841 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T227895 NZ_CP123227:92908-93180 [Pseudomonas amygdali pv. aesculi]
GTGCTAGTTGAGTGGCTTCCACAAGCTTTGGAGGCACTGGAGGAAATCGCCGGCTACCTAGCCGAGCGCAACCCTTATGC
TGCCGAGCAAATGCAGGCCACCCTTGAGGCCACGGTCGAGGCTCTCCCCCTGCACCCTTACATCCACCGTCCCGGCCGTG
TTCCAGGAACGCGCGAAGCGGTTGTGCATCCCAACTACCTGGTTGTCTACCGCGTCGGTACAGAGCGCATCAGCATCGTG
GACGTCCTGCACGCCCGCCAAGAGTACCCCTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT227895 WP_003083775.1 NZ_CP089236:c161346-161119 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT227895 NZ_CP123227:92733-92918 [Pseudomonas amygdali pv. aesculi]
ATGAGCATTCAACTTGACCCTCGCGTCTCGGAGTTCGAGACCCAGGAACAGGCCGACAATTACGACCGTTGGTTTCGCCA
GCGTATCGAGCACAGTTTGGCCGATCCGCGTCCACCCGTGCCGCACGATGAAGCAATGGCTCGCGTCAGAGCCATGATCG
AGGCAAAGCGCCGCCGTGCTAGTTGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9