Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 117758..118668 Replicon plasmid pK54798
Accession NZ_CP089235
Organism Klebsiella sp. KPN54798

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag LPQ25_RS26625 Protein ID WP_004026354.1
Coordinates 118198..118668 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag LPQ25_RS26620 Protein ID WP_004026357.1
Coordinates 117758..118201 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPQ25_RS26590 (LPQ25_26590) 112777..113298 + 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -
LPQ25_RS26595 (LPQ25_26595) 113295..114248 + 954 Protein_124 fec operon regulator FecR -
LPQ25_RS26600 (LPQ25_26600) 114335..116461 + 2127 WP_004213640.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
LPQ25_RS26605 (LPQ25_26605) 116536..116655 + 120 Protein_126 Fe3+-citrate ABC transporter substrate-binding protein -
LPQ25_RS26610 (LPQ25_26610) 116836..117063 + 228 WP_115385634.1 DUF2188 domain-containing protein -
LPQ25_RS26615 (LPQ25_26615) 117154..117657 + 504 Protein_128 DUF4113 domain-containing protein -
LPQ25_RS26620 (LPQ25_26620) 117758..118201 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
LPQ25_RS26625 (LPQ25_26625) 118198..118668 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
LPQ25_RS26630 (LPQ25_26630) 118817..119514 + 698 WP_223175001.1 IS1-like element IS1A family transposase -
LPQ25_RS26635 (LPQ25_26635) 120932..121549 - 618 WP_234599518.1 DUF1173 family protein -
LPQ25_RS26640 (LPQ25_26640) 121582..121848 - 267 WP_223175074.1 DUF1173 family protein -
LPQ25_RS26645 (LPQ25_26645) 122025..122519 + 495 WP_004212794.1 thermonuclease family protein -
LPQ25_RS26650 (LPQ25_26650) 122856..123254 + 399 WP_004212796.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 119011..119514 503
- inside Mobilizable plasmid - rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA / iroN / iroN / iroD / iroC / iroB 1..226890 226890


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T227893 WP_004026354.1 NZ_CP089235:118198-118668 [Klebsiella sp. KPN54798]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T227893 NZ_CP123226:9916-10293 [Pseudomonas amygdali pv. aesculi]
GTGAAAGGCGTTCTGGTAGACACCTCCGTTTGGGTTGAGCACTTTCGCAACAACAGTCCAGAACTGGTGAACTTGCTCAG
CCAAGACCGGGTACTCATACACCCCATGGTGATCGGAGAACTGGCGTGTGGTACGCCGCCTGATCGTTCGAACACCTTGA
CCGACCTTGGCGACCTACGAGGTGCACAACAACCCACGGTGAGTGAAGTCATCGCCTTTCTGAACACCCACAAGCTCTAT
GGCCTGGGCTGTGGGCTGGTGGATATGACGCTCCTGGCCTCCGCGTTATTGAGCGGCACAGCGCTTTGGACGTTAGACAA
GCGCCTGGAGCGTTTGGCTAGCCGCATGGCCGTGTCATACCAGCCTCCTACTCACTAG

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT227893 WP_004026357.1 NZ_CP089235:117758-118201 [Klebsiella sp. KPN54798]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT227893 NZ_CP123226:9719-9919 [Pseudomonas amygdali pv. aesculi]
ATGAGAACGACCGTCACCATCGACGATGCGTTGTACCGACGTGCTCTGGAAGTCGCCGATCCGGCGATGGATAAGGCCGA
CCTGTTCCGTGAAGCCGTTCAGACGTTTGTGCGCATTCAAGCGGCTAAACGATTGATGGCTTTAGGGGCCACACTGCCAG
CGATGGAAGACATCGCCCGCCGACACGAGAAGGCACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References