Detailed information of TA system TA227892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 65379..66049
• Replicon: plasmid pK54798
• Accession: NZ_CP089235
• Organism: Klebsiella sp. KPN54798

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: LPQ25_RS26320
• Protein ID: WP_004213072.1
• Coordinates: 65606..66049 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LPQ25_RS26315
• Protein ID: WP_004213073.1
• Coordinates: 65379..65609 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPQ25_RS26290 (LPQ25_26290)	61602..62501	-	900	WP_004225022.1	nucleotide-binding protein	-
LPQ25_RS26295 (LPQ25_26295)	62491..62781	-	291	WP_004213078.1	hypothetical protein	-
LPQ25_RS26300 (LPQ25_26300)	63133..63339	+	207	WP_004213077.1	hypothetical protein	-
LPQ25_RS26305 (LPQ25_26305)	63329..63622	-	294	WP_004213076.1	hypothetical protein	-
LPQ25_RS26310 (LPQ25_26310)	63638..64771	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LPQ25_RS26315 (LPQ25_26315)	65379..65609	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LPQ25_RS26320 (LPQ25_26320)	65606..66049	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
LPQ25_RS26325 (LPQ25_26325)	66198..66449	+	252	WP_186987481.1	hypothetical protein	-
LPQ25_RS26330 (LPQ25_26330)	66472..66776	-	305	Protein_71	transposase	-
LPQ25_RS26335 (LPQ25_26335)	67193..67830	+	638	Protein_72	mucoid phenotype regulator RmpA2	-
LPQ25_RS26340 (LPQ25_26340)	68348..68751	-	404	Protein_73	GAF domain-containing protein	-
LPQ25_RS26345 (LPQ25_26345)	68842..69762	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
LPQ25_RS26350 (LPQ25_26350)	69811..70302	-	492	WP_011251327.1	DM13 domain-containing protein	-
LPQ25_RS26355 (LPQ25_26355)	70365..70640	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA / iroN / iroN / iroD / iroC / iroB	1..226890	226890

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T227892 WP_004213072.1 NZ_CP089235:65606-66049 [Klebsiella sp. KPN54798]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T227892 NZ_CP123224:c1622-1329 [Pseudomonas amygdali pv. aesculi]
ATGCTACCCATTTTCTGGCTAGAATCAGCGGACAACGATTTGGCGGCAATAATCGAATACATAGGTTTACGTGATATAGC
TGCTGCCGAAAGGTTGTGGCAGCGCTTGCGTAGCATCGTTCTGCCCCTTTCAGAACATCCTTATCTGTATGCGATAAGTG
ACCGCGTTCCAGGAATGCGCGAGATCGTGGCCCATCCTAACTACCTGGTGTTTTATCGCGTCACCTCAACGCGCATAGAA
GTGGTGAACGTCGTCCATGCACGGCAAGAATATCCCCAAACTGGACTAGCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT227892 WP_004213073.1 NZ_CP089235:65379-65609 [Klebsiella sp. KPN54798]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT227892 NZ_CP123224:c1807-1622 [Pseudomonas amygdali pv. aesculi]
ATGATTGATCGTTCGCCTATCGTTTCGGAGTTTGAAACTGAAGAGCTAGAGGCCAATTACACCGCCTGGCTACGCGCGAA
GGTGGAGGCAAGTCTGGCCGACTCTCGCCCTGCTATCCCGCATGATGAAGTCGAGCGAAGGATGGCAGAGCGGTTGGCAC
GATTGCGCCATCGGCGAGCGTCCTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620