Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5342942..5343567 Replicon chromosome
Accession NZ_CP089234
Organism Klebsiella sp. KPN54798

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag LPQ25_RS25610 Protein ID WP_012737091.1
Coordinates 5342942..5343325 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LPQ25_RS25615 Protein ID WP_004150355.1
Coordinates 5343325..5343567 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPQ25_RS25595 (5340308) 5340308..5341210 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LPQ25_RS25600 (5341207) 5341207..5341842 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LPQ25_RS25605 (5341839) 5341839..5342768 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LPQ25_RS25610 (5342942) 5342942..5343325 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
LPQ25_RS25615 (5343325) 5343325..5343567 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LPQ25_RS25620 (5343772) 5343772..5344689 + 918 WP_012737090.1 alpha/beta hydrolase -
LPQ25_RS25625 (5344703) 5344703..5345644 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LPQ25_RS25630 (5345689) 5345689..5346126 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LPQ25_RS25635 (5346123) 5346123..5346983 - 861 WP_004146232.1 virulence factor BrkB family protein -
LPQ25_RS25640 (5346977) 5346977..5347576 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T227891 WP_012737091.1 NZ_CP089234:c5343325-5342942 [Klebsiella sp. KPN54798]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T227891 NZ_CP123223:22270-22596 [Pseudomonas amygdali pv. aesculi]
ATGATGCGAGGTGACCTAGTCACCGTCGCCATGCAAGGTGACTTTGGTAAACCAAGACCCGCCTTGGTAATTCAGGCAAA
CCAATTCAGCGAACACACCAGCGTTACCGTGCTGCCAGTGACCAGCACCCTAGTCGCAGCACCGCTGCTGCGCATCACCG
TTCAGCCGACCGCAGAAAACGGCTTGCAGAAGCCTTCTCAGGTCATGCTAGACAAGACCATGACACTCAAGCGTGAAAAA
ATAGGTCCGACTTTTGGGCATATCGGAGTGGATTCAATGGTGGAGGTGGAGCGCTGTTTAGCCGTGTTCTTGGGTATCGC
CAATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT227891 WP_004150355.1 NZ_CP089234:c5343567-5343325 [Klebsiella sp. KPN54798]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT227891 NZ_CP123223:22049-22273 [Pseudomonas amygdali pv. aesculi]
ATGCCTACATCCCATGTAAATGCCCGCGTTCAACGCCATCGCGACGCGCTGCGTGCGGCTGGCCTACGGCCCATTCAGAT
TTGGGTTCCCGATACCCGGCGGCCAGACTTTGCACAGGAGTGCCATCGACAGTGCCTGTTGGTTGCTCAGGCAGACATGG
CCAATACCTATATGCAGGGGTTGATGGACGAAGCCTTAGACGATGTTGAGGGTTGGACGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References