Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2740747..2742335 Replicon chromosome
Accession NZ_CP089234
Organism Klebsiella sp. KPN54798

Toxin (Protein)


Gene name hipA Uniprot ID A0A331XLA5
Locus tag LPQ25_RS13245 Protein ID WP_014907613.1
Coordinates 2740747..2742069 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag LPQ25_RS13250 Protein ID WP_002904790.1
Coordinates 2742069..2742335 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPQ25_RS13230 (2735803) 2735803..2738304 + 2502 WP_014907611.1 fimbria/pilus outer membrane usher protein -
LPQ25_RS13235 (2738292) 2738292..2739296 + 1005 WP_014907612.1 fimbrial protein -
LPQ25_RS13240 (2739438) 2739438..2740730 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
LPQ25_RS13245 (2740747) 2740747..2742069 - 1323 WP_014907613.1 type II toxin-antitoxin system HipA family toxin Toxin
LPQ25_RS13250 (2742069) 2742069..2742335 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
LPQ25_RS13255 (2742573) 2742573..2742899 - 327 WP_004215084.1 hydroxyisourate hydrolase -
LPQ25_RS13260 (2742896) 2742896..2743396 - 501 WP_004184022.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
LPQ25_RS13265 (2743374) 2743374..2744753 - 1380 WP_004184021.1 nucleobase:cation symporter-2 family protein -
LPQ25_RS13270 (2745099) 2745099..2746253 + 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
LPQ25_RS13275 (2746234) 2746234..2747157 - 924 WP_002904781.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49139.47 Da        Isoelectric Point: 7.5367

>T227888 WP_014907613.1 NZ_CP089234:c2742069-2740747 [Klebsiella sp. KPN54798]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T227888 NZ_CP123221:c2509357-2509067 [Pseudomonas amygdali pv. aesculi]
ATGACCTATAACCTCGAATTCGATGCGCGGGCTCTAAAGGAATGGCACAAATTAGGAGATACCGTGCGTCAGCAGCTCAA
GAAAAAGCTGGCTACGATTCTGGTTGCTCCGCGTGTTGAAGCCAATCGTCTGCATGCATTGCCTGACTGCTACAAAATCA
AACTGCGCAGTAGCGGCTATCGGCTGGTTTATCAGGTTATTGACCAAGAGGTTGTGGTCTTCGTGGTTGCGGTAGACAAG
CGTGAACGGGAAGAGGTTTATCGCAAGGCGACGGACCGGCTGGGTAGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT227888 WP_002904790.1 NZ_CP089234:c2742335-2742069 [Klebsiella sp. KPN54798]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT227888 NZ_CP123221:c2509586-2509347 [Pseudomonas amygdali pv. aesculi]
ATGGCGTCTATCAACGTTCGTATCGACGACGATCTCAAAGCGCGTGCTTACCTCGAGCTGGAAAAGCTTGGCGTCACCCC
CTCGGAGTTGCTGCGCCAAACCCTGCAGTACGTAGCTGAGCGTGGTCAGCTACCCTTCAAGACGGTTCTGATGACAGAGG
AAGACGAAGCGCTGTTGGCAACTGTGCGCGAGCGCCTTGCAGCTCCGCAGCGGGTCAAGGTTTCACTGGATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331XLA5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References