Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 327605..328191 Replicon chromosome
Accession NZ_CP089234
Organism Klebsiella sp. KPN54798

Toxin (Protein)


Gene name doc Uniprot ID A0A330WAT7
Locus tag LPQ25_RS01530 Protein ID WP_014906830.1
Coordinates 327823..328191 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag LPQ25_RS01525 Protein ID WP_004174006.1
Coordinates 327605..327826 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPQ25_RS01505 (323762) 323762..324688 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LPQ25_RS01510 (324685) 324685..325962 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
LPQ25_RS01515 (325959) 325959..326726 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
LPQ25_RS01520 (326728) 326728..327441 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
LPQ25_RS01525 (327605) 327605..327826 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LPQ25_RS01530 (327823) 327823..328191 + 369 WP_014906830.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LPQ25_RS01535 (328464) 328464..329780 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
LPQ25_RS01540 (329887) 329887..330774 + 888 WP_014906831.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
LPQ25_RS01545 (330771) 330771..331616 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
LPQ25_RS01550 (331618) 331618..332688 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 324685..333425 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(28-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13537.95 Da        Isoelectric Point: 8.6410

>T227877 WP_014906830.1 NZ_CP089234:327823-328191 [Klebsiella sp. KPN54798]
MTLQIISAEEIILFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T227877 NZ_CP123214:91619-91891 [Pseudomonas amygdali pv. aesculi]
GTGCTAGTTGAGTGGCTTCCACAAGCTTTGGAGGCACTGGAGGAAATCGCCGGCTACCTAGCCGAGCGCAACCCTTATGC
TGCCGAGCAAATGCAGGCCACCCTTGAGGCCACGGTCGAGGCTCTCCCCCTGCACCCTTACATCCACCGTCCCGGCCGTG
TTCCAGGAACGCGCGAAGCGGTTGTGCATCCCAACTACCTGGTTGTCTACCGCGTCGGTACAGAGCGCATCAGCATCGTG
GACGTCCTGCACGCCCGCCAAGAGTACCCCTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT227877 WP_004174006.1 NZ_CP089234:327605-327826 [Klebsiella sp. KPN54798]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT227877 NZ_CP123214:91444-91629 [Pseudomonas amygdali pv. aesculi]
ATGAGCATTCAACTTGACCCTCGCGTCTCGGAGTTCGAGACCCAGGAACAGGCCGACAATTACGACCGTTGGTTTCGCCA
GCGTATCGAGCACAGTTTGGCCGATCCGCGTCCACCCGTGCCGCACGATGAAGCAATGGCTCGCGTCAGAGCCATGATCG
AGGCAAAGCGCCGCCGTGCTAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330WAT7

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References