Detailed information of TA system TA227875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-RelB
• Location: 5981127..5981646
• Replicon: chromosome
• Accession: NZ_CP089227
• Organism: Nocardia asteroides strain FDAARGOS_1622

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U5EE58
• Locus tag: LT345_RS27665
• Protein ID: WP_019044609.1
• Coordinates: 5981377..5981646 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: U5E529
• Locus tag: LT345_RS27660
• Protein ID: WP_019044610.1
• Coordinates: 5981127..5981384 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT345_RS27635 (LT345_27635)	5976698..5978470	-	1773	WP_019044615.1	MDR family MFS transporter	-
LT345_RS27640 (LT345_27640)	5978467..5978964	-	498	WP_019044614.1	MarR family transcriptional regulator	-
LT345_RS27645 (LT345_27645)	5979054..5979875	-	822	WP_019044613.1	crotonase/enoyl-CoA hydratase family protein	-
LT345_RS27650 (LT345_27650)	5979983..5980459	+	477	WP_019044612.1	YbaK/EbsC family protein	-
LT345_RS27655 (LT345_27655)	5980528..5981079	+	552	WP_030204147.1	flavin reductase family protein	-
LT345_RS27660 (LT345_27660)	5981127..5981384	+	258	WP_019044610.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LT345_RS27665 (LT345_27665)	5981377..5981646	+	270	WP_019044609.1	Txe/YoeB family addiction module toxin	Toxin
LT345_RS27670 (LT345_27670)	5981668..5982981	-	1314	WP_019044608.1	aminotransferase class V-fold PLP-dependent enzyme	-
LT345_RS27675 (LT345_27675)	5983383..5983817	+	435	WP_019044607.1	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-66)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10122.61 Da
• Isoelectric Point: 9.3952

>T227875 WP_019044609.1 NZ_CP089227:5981377-5981646 [Nocardia asteroides]
MPERKLAFSAYGWETYTSWLVRDRTVLKAANKVIAAALADPFAGIGKPEPLKHHLAGHWSRRVTREHRMIYYVTDSEIVV
IAVGGHYDE

Nucleotide

• Length: 270 bp

>T227875 NZ_CP123213:c99987-99610 [Pseudomonas amygdali pv. aesculi]
GTGAAAGGCGTTCTGGTAGACACCTCCGTTTGGGTTGAGCACTTTCGCAACAACAGTCCAGAACTGGTGAACTTGCTCAG
CCAAGACCGGGTACTCATACACCCCATGGTGATCGGAGAACTGGCGTGTGGTACGCCGCCTGATCGTTCGAACACCTTGA
CCGACCTTGGCGACCTACGAGGTGCACAACAACCCACGGTGAGTGAAGTCATCGCCTTTCTGAACACCCACAAGCTCTAT
GGCCTGGGCTGTGGGCTGGTGGATATGACGCTCCTGGCCTCCGCGTTATTGAGCGGCACAGCGCTTTGGACGTTAGACAA
GCGCCTGGAGCGTTTGGCTAGCCGCATGGCCGTGTCATACCAGCCTCCTACTCACTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9375.41 Da
• Isoelectric Point: 4.4193

>AT227875 WP_019044610.1 NZ_CP089227:5981127-5981384 [Nocardia asteroides]
MTALPISTARQNLFKLVQQVNDDHDTVTVVTKSGENAVLVAESDWNSIQETLYVLATHGGVRLLESARDAREGRFEAHEL
IDPDA

Nucleotide

• Length: 258 bp

>AT227875 NZ_CP123213:c100184-99984 [Pseudomonas amygdali pv. aesculi]
ATGAGAACGACCGTCACCATCGACGATGCGTTGTACCGACGTGCTCTGGAAGTCGCCGATCCGGCGATGGATAAGGCCGA
CCTGTTCCGTGAAGCCGTTCAGACGTTTGTGCGCATTCAAGCGGCTAAACGATTGATGGCTTTAGGGGCCACACTGCCAG
CGATGGAAGACATCGCCCGCCGACACGAGAAGGCACTGTGA

Structures

Toxin

• AlphaFold DB: U5EE58

Antitoxin

• AlphaFold DB: U5E529