Detailed information of TA system TA227874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 5295378..5296014
• Replicon: chromosome
• Accession: NZ_CP089227
• Organism: Nocardia asteroides strain FDAARGOS_1622

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: U5EBJ0
• Locus tag: LT345_RS24580
• Protein ID: WP_019045202.1
• Coordinates: 5295607..5296014 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U5EM71
• Locus tag: LT345_RS24575
• Protein ID: WP_019045203.1
• Coordinates: 5295378..5295617 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT345_RS24540 (LT345_24540)	5290430..5291578	+	1149	WP_019045210.1	homoserine O-acetyltransferase	-
LT345_RS24545 (LT345_24545)	5291582..5292226	-	645	WP_019045209.1	TetR/AcrR family transcriptional regulator	-
LT345_RS24550 (LT345_24550)	5292378..5293340	+	963	WP_019045208.1	oxygenase MpaB family protein	-
LT345_RS24555 (LT345_24555)	5293378..5293755	-	378	WP_019045207.1	helix-turn-helix domain-containing protein	-
LT345_RS24560 (LT345_24560)	5293825..5294472	+	648	WP_019045206.1	nitroreductase family protein	-
LT345_RS24565 (LT345_24565)	5294465..5294710	-	246	WP_232017306.1	DUF3017 domain-containing protein	-
LT345_RS24570 (LT345_24570)	5294880..5295344	+	465	WP_019045204.1	tRNA (cytidine(34)-2'-O)-methyltransferase	-
LT345_RS24575 (LT345_24575)	5295378..5295617	+	240	WP_019045203.1	hypothetical protein	Antitoxin
LT345_RS24580 (LT345_24580)	5295607..5296014	+	408	WP_019045202.1	type II toxin-antitoxin system VapC family toxin	Toxin
LT345_RS24585 (LT345_24585)	5296016..5296243	-	228	WP_019045201.1	DUF167 domain-containing protein	-
LT345_RS24590 (LT345_24590)	5296203..5297165	-	963	WP_022567285.1	DUF4349 domain-containing protein	-
LT345_RS24595 (LT345_24595)	5297225..5298211	-	987	WP_019045199.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (7-133)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14745.93 Da
• Isoelectric Point: 6.6367

>T227874 WP_019045202.1 NZ_CP089227:5295607-5296014 [Nocardia asteroides]
VASDEYVLDGSAASTALLRKDAVGIAVGRLIETSTCHAPHLIDAEVGNVLRRHERKGLVDPETATIGLRMLATMVDRRYA
HQGWLSEQAWLLRHTITFYDGLYAALAARLDVPLLTSDARLSKAPGLPCRVEVID

Nucleotide

• Length: 408 bp

>T227874 NZ_CP123213:c1622-1329 [Pseudomonas amygdali pv. aesculi]
ATGCTACCCATTTTCTGGCTAGAATCAGCGGACAACGATTTGGCGGCAATAATCGAATACATAGGTTTACGTGATATAGC
TGCTGCCGAAAGGTTGTGGCAGCGCTTGCGTAGCATCGTTCTGCCCCTTTCAGAACATCCTTATCTGTATGCGATAAGTG
ACCGCGTTCCAGGAATGCGCGAGATCGTGGCCCATCCTAACTACCTGGTGTTTTATCGCGTCACCTCAACGCGCATAGAA
GTGGTGAACGTCGTCCATGCACGGCAAGAATATCCCCAAACTGGACTAGCGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8806.83 Da
• Isoelectric Point: 4.7064

>AT227874 WP_019045203.1 NZ_CP089227:5295378-5295617 [Nocardia asteroides]
MATIQIRDIPDEDAEVFRRRAEAEGMSLQAYMRRELIAAARRRSKTEALAAVREALERDSSAGADRESILSALDDARGE

Nucleotide

• Length: 240 bp

>AT227874 NZ_CP123213:c1807-1622 [Pseudomonas amygdali pv. aesculi]
ATGATTGATCGTTCGCCTATCGTTTCGGAGTTTGAAACTGAAGAGCTAGAGGCCAATTACACCGCCTGGCTACGCGCGAA
GGTGGAGGCAAGTCTGGCCGACTCTCGCCCTGCTATCCCGCATGATGAAGTCGAGCGAAGGATGGCAGAGCGGTTGGCAC
GATTGCGCCATCGGCGAGCGTCCTGA

Structures

Toxin

• AlphaFold DB: U5EBJ0

Antitoxin

• AlphaFold DB: U5EM71