Detailed information of TA system TA227871

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 3163886..3164412
• Replicon: chromosome
• Accession: NZ_CP089225
• Organism: Nocardia gipuzkoensis strain FDAARGOS_1619

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LTT66_RS14320
• Protein ID: WP_233067576.1
• Coordinates: 3164155..3164412 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LTT66_RS14315
• Protein ID: WP_269822012.1
• Coordinates: 3163886..3164158 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTT66_RS14280 (LTT66_14280)	3158916..3159263	+	348	WP_195104106.1	spore germination protein GerW family protein	-
LTT66_RS14285 (LTT66_14285)	3159343..3159534	-	192	WP_233067570.1	DUF1918 domain-containing protein	-
LTT66_RS14290 (LTT66_14290)	3159599..3160192	-	594	WP_195066902.1	TetR/AcrR family transcriptional regulator	-
LTT66_RS14295 (LTT66_14295)	3160251..3160826	+	576	WP_233069244.1	dihydrofolate reductase family protein	-
LTT66_RS14300 (LTT66_14300)	3160915..3161328	-	414	WP_233067573.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
LTT66_RS14305 (LTT66_14305)	3161492..3162757	+	1266	WP_181721103.1	aspartate kinase	-
LTT66_RS14310 (LTT66_14310)	3162761..3163795	+	1035	WP_181721104.1	aspartate-semialdehyde dehydrogenase	-
LTT66_RS14315 (LTT66_14315)	3163886..3164158	+	273	WP_269822012.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LTT66_RS14320 (LTT66_14320)	3164155..3164412	+	258	WP_233067576.1	Txe/YoeB family addiction module toxin	Toxin
LTT66_RS14325 (LTT66_14325)	3164474..3165397	+	924	WP_233067578.1	aminotransferase class IV	-
LTT66_RS14330 (LTT66_14330)	3165412..3166761	-	1350	WP_233067581.1	serine hydrolase	-
LTT66_RS14335 (LTT66_14335)	3166856..3168097	-	1242	WP_233067583.1	APC family permease	-
LTT66_RS14340 (LTT66_14340)	3168636..3169061	+	426	WP_233067585.1	Fur family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (12-77)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9950.49 Da
• Isoelectric Point: 7.3158

>T227871 WP_233067576.1 NZ_CP089225:3164155-3164412 [Nocardia gipuzkoensis]
MKAVFSEAAWEEYSAWLQRDRLVLKKVNVLIEAILADPFEGIGKPEPLKHDLAGYWSRRITQEHRIVYRIVDDAVWIAKV
GKHYD

Nucleotide

• Length: 258 bp

>T227871 NZ_CP123210:42418-42744 [Pseudomonas amygdali pv. aesculi]
ATGATGCGAGGTGACCTAGTCACCGTCGCCATGCAAGGTGACTTTGGTAAACCAAGACCCGCCTTGGTAATTCAGGCAAA
CCAATTCAGCGAACACACCAGCGTTACCGTGCTGCCAGTGACCAGCACCCTAGTCGCAGCACCGCTGCTGCGCATCACCG
TTCAGCCGACCGCAGAAAACGGCTTGCAGAAGCCTTCTCAGGTCATGCTAGACAAGACCATGACACTCAAGCGTGAAAAA
ATAGGTCCGACTTTTGGGCATATCGGAGTGGATTCAATGGTGGAGGTGGAGCGCTGTTTAGCCGTGTTCTTGGGTATCGC
CAATTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10008.38 Da
• Isoelectric Point: 4.4805

>AT227871 WP_269822012.1 NZ_CP089225:3163886-3164158 [Nocardia gipuzkoensis]
MYYWLGGMMASAITASEARRNLFPLIRMVNEDHDTIEIVSKGGNAVLMSKEDYDSLMTTVELLSGRDGARLRRSIQELEG
GGGVERDLIE

Nucleotide

• Length: 273 bp

>AT227871 NZ_CP123210:42197-42421 [Pseudomonas amygdali pv. aesculi]
ATGCCTACATCCCATGTAAATGCCCGCGTTCAACGCCATCGCGACGCGCTGCGTGCGGCTGGCCTACGGCCCATTCAGAT
TTGGGTTCCCGATACCCGGCGGCCAGACTTTGCACAGGAGTGCCATCGACAGTGCCTGTTGGTTGCTCAGGCAGACATGG
CCAATACCTATATGCAGGGGTTGATGGACGAAGCCTTAGACGATGTTGAGGGTTGGACGCCATGA