Detailed information of TA system TA227870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3007337..3007968
• Replicon: chromosome
• Accession: NZ_CP089225
• Organism: Nocardia gipuzkoensis strain FDAARGOS_1619

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LTT66_RS13600
• Protein ID: WP_233067374.1
• Coordinates: 3007552..3007968 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LTT66_RS13595
• Protein ID: WP_233067373.1
• Coordinates: 3007337..3007555 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTT66_RS13585 (LTT66_13585)	3003176..3003673	-	498	WP_181723286.1	peptidylprolyl isomerase	-
LTT66_RS13590 (LTT66_13590)	3003873..3007229	+	3357	WP_233067370.1	arabinosyltransferase domain-containing protein	-
LTT66_RS13595 (LTT66_13595)	3007337..3007555	+	219	WP_233067373.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LTT66_RS13600 (LTT66_13600)	3007552..3007968	+	417	WP_233067374.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTT66_RS13605 (LTT66_13605)	3008124..3008282	-	159	WP_168434190.1	hypothetical protein	-
LTT66_RS13610 (LTT66_13610)	3008369..3008770	-	402	WP_181723283.1	helix-turn-helix domain-containing protein	-
LTT66_RS13615 (LTT66_13615)	3008868..3009791	+	924	WP_233067376.1	NADP-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (8-131)

Antitoxin

• Post-segregation antitoxin CcdA (3-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14966.10 Da
• Isoelectric Point: 6.2367

>T227870 WP_233067374.1 NZ_CP089225:3007552-3007968 [Nocardia gipuzkoensis]
VTDSEQIVLDASAMVDILVRNDRTAAVISRITTTVMHVPAHFDAEVLSALGRLNRAGDLTDGDVETALSRLARAPLTRHP
LLNLTKGAWARRAAIRLTDALYVELAEQLDMPLITTDGRLARASSRAEGIRAAAELRE

Nucleotide

• Length: 417 bp

>T227870 NZ_CP123209:c4180784-4180494 [Pseudomonas amygdali pv. aesculi]
ATGACCTATAACCTCGAATTCGATGCGCGGGCTCTAAAGGAATGGCACAAATTAGGAGATACCGTGCGTCAGCAGCTCAA
GAAAAAGCTGGCTACGATTCTGGTTGCTCCGCGTGTTGAAGCCAATCGTCTGCATGCATTGCCTGACTGCTACAAAATCA
AACTGCGCAGTAGCGGCTATCGGCTGGTTTATCAGGTTATTGACCAAGAGGTTGTGGTCTTCGTGGTTGCGGTAGACAAG
CGTGAACGGGAAGAGGTTTATCGCAAGGCGACGGACCGGCTGGGTAGGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 7884.78 Da
• Isoelectric Point: 4.2950

>AT227870 WP_233067373.1 NZ_CP089225:3007337-3007555 [Nocardia gipuzkoensis]
MTRLNVYVPDELAERARERGLNVSALTQAAILAALEKSATSAWLAEFDDDRPEVSHEAVLAAIDAARDEFGR

Nucleotide

• Length: 219 bp

>AT227870 NZ_CP123209:c4181013-4180774 [Pseudomonas amygdali pv. aesculi]
ATGGCGTCTATCAACGTTCGTATCGACGACGATCTCAAAGCGCGTGCTTACCTCGAGCTGGAAAAGCTTGGCGTCACCCC
CTCGGAGTTGCTGCGCCAAACCCTGCAGTACGTAGCTGAGCGTGGTCAGCTACCCTTCAAGACGGTTCTGATGACAGAGG
AAGACGAAGCGCTGTTGGCAACTGTGCGCGAGCGCCTTGCAGCTCCGCAGCGGGTCAAGGTTTCACTGGATGACCTATAA