Detailed information of TA system TA227869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 507422..508065
• Replicon: chromosome
• Accession: NZ_CP089225
• Organism: Nocardia gipuzkoensis strain FDAARGOS_1619

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LTT66_RS02330
• Protein ID: WP_233068960.1
• Coordinates: 507673..508065 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LTT66_RS02325
• Protein ID: WP_233063491.1
• Coordinates: 507422..507676 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTT66_RS02310 (LTT66_02310)	503069..503887	-	819	WP_195065132.1	PhzF family phenazine biosynthesis protein	-
LTT66_RS02315 (LTT66_02315)	503969..506392	-	2424	WP_233063489.1	HAD-IC family P-type ATPase	-
LTT66_RS02320 (LTT66_02320)	506428..507366	+	939	WP_233063490.1	EamA family transporter RarD	-
LTT66_RS02325 (LTT66_02325)	507422..507676	+	255	WP_233063491.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LTT66_RS02330 (LTT66_02330)	507673..508065	+	393	WP_233068960.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTT66_RS02335 (LTT66_02335)	508134..508634	-	501	WP_233063493.1	hypothetical protein	-
LTT66_RS02340 (LTT66_02340)	508830..510158	-	1329	WP_233068961.1	DNA polymerase IV	-
LTT66_RS02345 (LTT66_02345)	510562..511449	+	888	WP_181724539.1	UTP--glucose-1-phosphate uridylyltransferase	-
LTT66_RS02350 (LTT66_02350)	511586..512137	-	552	WP_233063495.1	NlpC/P60 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-47)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14456.55 Da
• Isoelectric Point: 4.7051

>T227869 WP_233068960.1 NZ_CP089225:507673-508065 [Nocardia gipuzkoensis]
VNMQRALADTSIFIGIEAERFDPTRFDGYEWGVSVVTLGELRLGVLRASSPEVSARRLATYQLAQRFQPLELDEAVAEYW
ALLVARLRAAGRKVPINDSWIAATALAHDIPIVTQDGDYDAMPDLKVLKL

Nucleotide

• Length: 393 bp

>T227869 NZ_CP123207:c54256-53990 [Pseudomonas amygdali pv. aesculi]
TTGGCCTGGACGATTGATTACACCGACACGGCCAAAACACAGTTGCGGAAGCTCGACAAGCAGTCAGCGAAGCGCATTCT
TGACTTTATGGATGAACGGGTGTCCAGCAGGGACGATCCGAGAACTACTGGCAAGGCGCTAACAGGCCCATTGGGGGGCT
TGTGGCGGTATCGCGTAGGAGACTTCCGGGTAATCTGCGAGATACAGGACGGCGCCTTACGGATACTGGTTGTACAGCTT
GGCAACCGTCGAGAAGTTTACCGCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9720.14 Da
• Isoelectric Point: 5.2082

>AT227869 WP_233063491.1 NZ_CP089225:507422-507676 [Nocardia gipuzkoensis]
MTALPARELRNHTAEVLRRVEAGEEIEILKDNRPVAKIIPLPRRRQWVPASEVLRELVRLGPDTTGLREELRETLTDTTD
DLPW

Nucleotide

• Length: 255 bp

>AT227869 NZ_CP123207:c54467-54240 [Pseudomonas amygdali pv. aesculi]
ATGGCCACTTCCATTCGCTTGGCACCCGAAACCGAGCAGCGCCTAGATTTTTTGGCGACAAGCACCGGGCGTACCAAGGC
GTATTATCTGCGCGAAATCATCGATCATGGCCTGACTGACCTTGAGGATTACTACCTCGCGGCTGAAGTCCTGGAGCGAG
TCCGTAAAAATACAGAGGTCGTTCACTCTGCTGCGGACGTAAGGAAAGACCTTGGCCTGGACGATTGA