Detailed information of TA system TA227868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 6119737..6120422
• Replicon: chromosome
• Accession: NZ_CP089224
• Organism: Mycobacterium ostraviense strain FDAARGOS_1613

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A162D6L3
• Locus tag: LTS72_RS28100
• Protein ID: WP_075510149.1
• Coordinates: 6119737..6120168 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LTS72_RS28105
• Protein ID: WP_075510148.1
• Coordinates: 6120165..6120422 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTS72_RS28080 (LTS72_28080)	6115635..6116582	+	948	WP_075510153.1	bifunctional MaoC family dehydratase N-terminal/OB-fold nucleic acid binding domain-containing protein	-
LTS72_RS28085 (LTS72_28085)	6116579..6116980	+	402	WP_075510152.1	MaoC family dehydratase	-
LTS72_RS28090 (LTS72_28090)	6116980..6118152	+	1173	WP_075510151.1	lipid-transfer protein	-
LTS72_RS28095 (LTS72_28095)	6118177..6119643	-	1467	WP_075510150.1	PE family protein	-
LTS72_RS28100 (LTS72_28100)	6119737..6120168	-	432	WP_075510149.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTS72_RS28105 (LTS72_28105)	6120165..6120422	-	258	WP_075510148.1	CopG family transcriptional regulator	Antitoxin
LTS72_RS28110 (LTS72_28110)	6120570..6121409	+	840	WP_075510147.1	hypothetical protein	-
LTS72_RS28115 (LTS72_28115)	6121448..6122329	-	882	WP_075510146.1	MaoC/PaaZ C-terminal domain-containing protein	-
LTS72_RS28120 (LTS72_28120)	6122331..6124025	-	1695	WP_075510266.1	3-oxosteroid 1-dehydrogenase	-
LTS72_RS28125 (LTS72_28125)	6124109..6124894	+	786	WP_075510145.1	2-keto-4-pentenoate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-133)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15623.73 Da
• Isoelectric Point: 4.8576

>T227868 WP_075510149.1 NZ_CP089224:c6120168-6119737 [Mycobacterium ostraviense]
MKLLDLNLLIYAIDESTARHEAAREWLDDTLSGSATVAFAWHVLVGFVRLSTRAAIFERPLTVDQSFDIVDGWLAQPCVT
VAHPTDRHATVLRGLLTPLGTAGNLTSDAHLAALAVEHGAELCSTDVDFTRFSGVRWIDPLQT

Nucleotide

• Length: 432 bp

>T227868 NZ_CP123207:c23873-23547 [Pseudomonas amygdali pv. aesculi]
ATGATGCGAGGTGACCTAGTCACCGTCGCCATGCAAGGTGACTTTGGTAAACCAAGACCCGCCTTGGTAATTCAGGCAAA
CCAATTCAGCGAACACACCAGCGTTACCGTGCTGCCAGTGACCAGCACCCTAGTCGCAGCACCGCTGCTGCGCATCACCG
TTCAGCCGACCGCAGAAAACGGCTTGCAGAAGCCTTCTCAGGTCATGCTAGACAAGACCATGACACTCAAGCGTGAAAAA
ATAGGTCCGACTTTTGGGCATATCGGAGTGGATTCAATGGTGGAGGTGGAGCGCTGTTTAGCCGTGTTCTTGGGTATCGC
CAATTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9333.69 Da
• Isoelectric Point: 11.3519

>AT227868 WP_075510148.1 NZ_CP089224:c6120422-6120165 [Mycobacterium ostraviense]
VRTTVTLDPDVVAALQRAVRERGMSFKAVLNDAVRRGLSGESTRRRYRTPSRDLGLRAGVDIDKALALAAGDEDAETLRK
LALRK

Nucleotide

• Length: 258 bp

>AT227868 NZ_CP123207:c24094-23870 [Pseudomonas amygdali pv. aesculi]
ATGCCTACATCCCATGTAAATGCCCGCGTTCAACGCCATCGCGACGCGCTGCGTGCGGCTGGCCTACGGCCCATTCAGAT
TTGGGTTCCCGATACCCGGCGGCCAGACTTTGCACAGGAGTGCCATCGACAGTGCCTGTTGGTTGCTCAGGCAGACATGG
CCAATACCTATATGCAGGGGTTGATGGACGAAGCCTTAGACGATGTTGAGGGTTGGACGCCATGA

Structures

Toxin

• AlphaFold DB: A0A162D6L3