Detailed information of TA system TA227867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 5753463..5754006
• Replicon: chromosome
• Accession: NZ_CP089224
• Organism: Mycobacterium ostraviense strain FDAARGOS_1613

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A164EQC9
• Locus tag: LTS72_RS26165
• Protein ID: WP_075509186.1
• Coordinates: 5753463..5753774 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LTS72_RS26170
• Protein ID: WP_082276404.1
• Coordinates: 5753761..5754006 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTS72_RS26140 (LTS72_26140)	5748630..5750318	-	1689	WP_233026884.1	IS607 family element RNA-guided endonuclease TnpB	-
LTS72_RS26145 (LTS72_26145)	5750319..5750903	-	585	WP_082276364.1	IS607 family transposase	-
LTS72_RS26150 (LTS72_26150)	5751091..5751960	+	870	WP_075509185.1	BglII/BstYI family type II restriction endonuclease	-
LTS72_RS26155 (LTS72_26155)	5752098..5752964	+	867	WP_233026885.1	MT-A70 family methyltransferase	-
LTS72_RS26160 (LTS72_26160)	5753096..5753233	-	138	WP_161951580.1	hypothetical protein	-
LTS72_RS26165 (LTS72_26165)	5753463..5753774	-	312	WP_075509186.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LTS72_RS26170 (LTS72_26170)	5753761..5754006	-	246	WP_082276404.1	ribbon-helix-helix domain-containing protein	Antitoxin
LTS72_RS26175 (LTS72_26175)	5754125..5755444	-	1320	WP_075509188.1	serpin family protein	-
LTS72_RS26180 (LTS72_26180)	5755568..5756083	-	516	WP_075509189.1	ester cyclase	-
LTS72_RS26185 (LTS72_26185)	5756320..5757318	-	999	WP_082276934.1	phosphotransferase family protein	-
LTS72_RS26190 (LTS72_26190)	5757369..5757755	+	387	WP_075509194.1	nuclear transport factor 2 family protein	-
LTS72_RS26195 (LTS72_26195)	5757752..5758099	-	348	WP_075509191.1	nuclear transport factor 2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5750319..5750828	509

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11230.07 Da
• Isoelectric Point: 11.6876

>T227867 WP_075509186.1 NZ_CP089224:c5753774-5753463 [Mycobacterium ostraviense]
MRRGELWFAATPGGDRPVLILTRDPVADRIGAVVVAALTRTRRGLVSELALTAADDLLPSDCVVNFDNIHTLPRTALRRQ
ITRLSPARMHQVCRTLRAATGCG

Nucleotide

• Length: 312 bp

>T227867 NZ_CP123206:c68503-68126 [Pseudomonas amygdali pv. aesculi]
GTGAAAGGCGTTCTGGTAGACACCTCCGTTTGGGTTGAGCACTTTCGCAACAACAGTCCAGAACTGGTGAACTTGCTCAG
CCAAGACCGGGTACTCATACACCCCATGGTGATCGGAGAACTGGCGTGTGGTACGCCGCCTGATCGTTCGAACACCTTGA
CCGACCTTGGCGACCTACGAGGTGCACAACAACCCACGGTGAGTGAAGTCATCGCCTTTCTGAACACCCACAAGCTCTAT
GGCCTGGGCTGTGGGCTGGTGGATATGACGCTCCTGGCCTCCGCGTTATTGAGCGGCACAGCGCTTTGGACGTTAGACAA
GCGCCTGGAGCGTTTGGCTAGCCGCATGGCCGTGTCATACCAGCCTCCTACTCACTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9247.07 Da
• Isoelectric Point: 4.0794

>AT227867 WP_082276404.1 NZ_CP089224:c5754006-5753761 [Mycobacterium ostraviense]
MVSFRVDDEDAAAVEQWARRLHIDRSELLRQALRRHLAELAADQDVQAYAEQPLTDDEKALGDIADWGPAEDWTDWADAA
R

Nucleotide

• Length: 246 bp

>AT227867 NZ_CP123206:c68700-68500 [Pseudomonas amygdali pv. aesculi]
ATGAGAACGACCGTCACCATCGACGATGCGTTGTACCGACGTGCTCTGGAAGTCGCCGATCCGGCGATGGATAAGGCCGA
CCTGTTCCGTGAAGCCGTTCAGACGTTTGTGCGCATTCAAGCGGCTAAACGATTGATGGCTTTAGGGGCCACACTGCCAG
CGATGGAAGACATCGCCCGCCGACACGAGAAGGCACTGTGA

Structures

Toxin

• AlphaFold DB: A0A164EQC9