227866

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 5162690..5163266 Replicon chromosome
Accession NZ_CP089224
Organism Mycobacterium ostraviense strain FDAARGOS_1613

Toxin (Protein)


Gene name mazF Uniprot ID A0A163X2A1
Locus tag LTS72_RS23505 Protein ID WP_075512457.1
Coordinates 5162690..5163037 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A163X2B3
Locus tag LTS72_RS23510 Protein ID WP_075512458.1
Coordinates 5163030..5163266 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LTS72_RS23485 (LTS72_23485) 5157803..5159431 - 1629 WP_180276415.1 acetolactate synthase large subunit -
LTS72_RS23490 (LTS72_23490) 5159519..5160892 + 1374 WP_075512455.1 NADP-dependent succinic semialdehyde dehydrogenase -
LTS72_RS23495 (LTS72_23495) 5160971..5161915 - 945 WP_023369766.1 R2-like ligand-binding oxidase -
LTS72_RS23500 (LTS72_23500) 5161912..5162595 - 684 WP_075512456.1 TetR/AcrR family transcriptional regulator -
LTS72_RS23505 (LTS72_23505) 5162690..5163037 - 348 WP_075512457.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LTS72_RS23510 (LTS72_23510) 5163030..5163266 - 237 WP_075512458.1 antitoxin Antitoxin
LTS72_RS23515 (LTS72_23515) 5163453..5163593 + 141 WP_161951676.1 hypothetical protein -
LTS72_RS28425 5163804..5164169 - 366 WP_142361277.1 PE family protein -
LTS72_RS23530 (LTS72_23530) 5164093..5165811 - 1719 WP_075512460.1 acyl-CoA dehydrogenase -
LTS72_RS23535 (LTS72_23535) 5165949..5166929 + 981 WP_075512461.1 phosphotriesterase -
LTS72_RS23540 (LTS72_23540) 5166950..5168119 - 1170 WP_075512583.1 acyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12362.40 Da        Isoelectric Point: 7.1697

>T227866 WP_075512457.1 NZ_CP089224:c5163037-5162690 [Mycobacterium ostraviense]
MAEPRRGELWLVALGAARAAEPGKHRPAVVVSVDEILTGIDDELIVVVPVSSSLARSPLRPDISPAEGVEAASVAVCRSI
RAISRTRLLRRLGTLAPDTMRQIEHALELILGIDR

Download         Length: 348 bp

>T227866 NZ_CP123205:100417-100767 [Pseudomonas amygdali pv. aesculi]
ATGAACACGTTTGCACTACGCTTTACCGAGGTTGCCCAGCAAAGCATTGAAGATCAGGTCGAGCACCTGGCTATCACTCA
GGGATTCTCATTAGCTGCTCAACGTATAAATACCCTGATCGATGTCATCCAGGACAAGCTCTTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCTCAATTGAGTGAGCTGGGTATACTGCACTATCGTGAGTTGAACACTGACGGATACCGGATCTTTTAC
GAGGTCATGGATTCTGATGGTATTACCGACATCGCCGTATTGCTCGTATTAGGCGGGAAACAAAGTGTGGAGCAGGCTCT
GATCCGCTACTGCCTGCTCCAGCCGATGTAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8675.60 Da        Isoelectric Point: 6.4685

>AT227866 WP_075512458.1 NZ_CP089224:c5163266-5163030 [Mycobacterium ostraviense]
VSTSRTTIRVSVQTRDRLATQARERGLSISALLAELAARLEREAIFRAEREATRAEATSQAVHDEHRDWDITVSDGLG

Download         Length: 237 bp

>AT227866 NZ_CP123205:100156-100404 [Pseudomonas amygdali pv. aesculi]
ATGCGAGTTGAAACAATAAGTTATCTGAAACGTAACGCAGCCGACCTGCCTCTCGATGAGCCACTGGTTGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCAGATCGTAAGCGCCGCGATGAGTCGATCGCCTTGGTAAAGCTCCTGG
CGATCAGTTCCCGCGATTACTCCCAAGGCAAGCACTGTTCCACTGACGAACTTAAAGCCCGGTTGAGCAGGAGGTTTGCC
CAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A163X2A1

Antitoxin

Source ID Structure
AlphaFold DB A0A163X2B3

References