Detailed information of TA system TA227862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 4399295..4399940
• Replicon: chromosome
• Accession: NZ_CP089224
• Organism: Mycobacterium ostraviense strain FDAARGOS_1613

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A164A3F9
• Locus tag: LTS72_RS19915
• Protein ID: WP_075510949.1
• Coordinates: 4399524..4399940 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LTS72_RS19910
• Protein ID: WP_036411107.1
• Coordinates: 4399295..4399516 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTS72_RS19895 (LTS72_19895)	4395916..4396479	-	564	WP_082277149.1	helix-turn-helix domain-containing protein	-
LTS72_RS19900 (LTS72_19900)	4396537..4397427	+	891	WP_075510951.1	TauD/TfdA family dioxygenase	-
LTS72_RS19905 (LTS72_19905)	4397436..4399181	-	1746	WP_075510950.1	GMC family oxidoreductase	-
LTS72_RS19910 (LTS72_19910)	4399295..4399516	+	222	WP_036411107.1	hypothetical protein	Antitoxin
LTS72_RS19915 (LTS72_19915)	4399524..4399940	+	417	WP_075510949.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTS72_RS19920 (LTS72_19920)	4400013..4401152	-	1140	WP_075510948.1	GuaB3 family IMP dehydrogenase-related protein	-
LTS72_RS19925 (LTS72_19925)	4401162..4402781	-	1620	WP_075511025.1	IMP dehydrogenase	-
LTS72_RS19930 (LTS72_19930)	4402958..4403371	+	414	WP_023371174.1	DUF5319 domain-containing protein	-
LTS72_RS19935 (LTS72_19935)	4403395..4404279	-	885	WP_075510947.1	anti-sigma-D factor RsdA	-
LTS72_RS19940 (LTS72_19940)	4404272..4404847	-	576	WP_082277148.1	ECF RNA polymerase sigma factor SigD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14935.26 Da
• Isoelectric Point: 7.4785

>T227862 WP_075510949.1 NZ_CP089224:4399524-4399940 [Mycobacterium ostraviense]
VALLDVNALVALAWDSHIHHVRIREWFAANASRGWATCPITESGFVRVSTNPKVLPSPIGIADARRMLAALRAVDGHRFL
VDDVSPVDGDVPAIVGHRQVTDAHLLALARRRGVRLLTFDAALVALGEGRDVELLTPL

Nucleotide

• Length: 417 bp

>T227862 NZ_CP123199:3612-3992 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGATATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTAGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCCCCTGAACTGGCAGACCTTACTGTAGGGGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 7904.00 Da
• Isoelectric Point: 4.7420

>AT227862 WP_036411107.1 NZ_CP089224:4399295-4399516 [Mycobacterium ostraviense]
VRTTLDIDDDVVAAARELAASQRRSLGAVISELARRGLTPARVETDDKLPVIRVPAGTPPITPEMVRRALDED

Nucleotide

• Length: 222 bp

>AT227862 NZ_CP123199:3391-3612 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A164A3F9