Detailed information of TA system TA227860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3271544..3272175
• Replicon: chromosome
• Accession: NZ_CP089224
• Organism: Mycobacterium ostraviense strain FDAARGOS_1613

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A164A8Q8
• Locus tag: LTS72_RS14915
• Protein ID: WP_075510855.1
• Coordinates: 3271544..3271942 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LTS72_RS14920
• Protein ID: WP_036417665.1
• Coordinates: 3271939..3272175 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTS72_RS14900 (LTS72_14900)	3268047..3269723	-	1677	WP_075510854.1	ribonuclease J	-
LTS72_RS14905 (LTS72_14905)	3269743..3270645	-	903	WP_075510873.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LTS72_RS14910 (LTS72_14910)	3270713..3271465	-	753	WP_075510874.1	FAD-dependent thymidylate synthase	-
LTS72_RS14915 (LTS72_14915)	3271544..3271942	-	399	WP_075510855.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTS72_RS14920 (LTS72_14920)	3271939..3272175	-	237	WP_036417665.1	hypothetical protein	Antitoxin
LTS72_RS14925 (LTS72_14925)	3272260..3275456	-	3197	Protein_2958	type I restriction endonuclease subunit R	-
LTS72_RS14930 (LTS72_14930)	3275440..3275826	-	387	WP_233027156.1	DUF1016 N-terminal domain-containing protein	-
LTS72_RS14935 (LTS72_14935)	3275717..3276181	-	465	WP_233026746.1	DUF1016 N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14514.84 Da
• Isoelectric Point: 9.4958

>T227860 WP_075510855.1 NZ_CP089224:c3271942-3271544 [Mycobacterium ostraviense]
VNDYVIDASALVLALAGKTKAADRLRARLPGMRRHAPQLIDAEVGNVLRRHELAGLISTREAETAARVASLLIDDRYPHV
GVLARRAWTLRHTMTFYDALYVALASYLDIPLLTGDARLSRAPGLICQTELV

Nucleotide

• Length: 399 bp

>T227860 NZ_CP123197:4602430-4602660 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8753.85 Da
• Isoelectric Point: 5.1231

>AT227860 WP_036417665.1 NZ_CP089224:c3272175-3271939 [Mycobacterium ostraviense]
MATIQIREIPDEAYEVIRKRARAAGRSIQSYMRDWVIQFASRPTTDEALAAMEAAREASETPGATRESILTDLAADRR

Nucleotide

• Length: 237 bp

>AT227860 NZ_CP123197:4602657-4603016 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A164A8Q8