Detailed information of TA system TA227853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3374868..3375432
• Replicon: chromosome
• Accession: NZ_CP089223
• Organism: Mycobacterium avium subsp. avium strain FDAARGOS_1609

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7VBD8
• Locus tag: LTQ57_RS15610
• Protein ID: WP_003875391.1
• Coordinates: 3374868..3375239 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LTQ57_RS15615
• Protein ID: WP_011724021.1
• Coordinates: 3375226..3375432 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTQ57_RS15565 (LTQ57_15565)	3370034..3370357	-	324	WP_009975396.1	putative quinol monooxygenase	-
LTQ57_RS15570 (LTQ57_15570)	3370768..3371292	-	525	WP_033713819.1	glycoside hydrolase	-
LTQ57_RS15575 (LTQ57_15575)	3371449..3371802	-	354	WP_003875396.1	hypothetical protein	-
LTQ57_RS15580 (LTQ57_15580)	3371872..3372255	-	384	WP_023884114.1	hypothetical protein	-
LTQ57_RS15585 (LTQ57_15585)	3372324..3372722	-	399	WP_009975395.1	VOC family protein	-
LTQ57_RS15590 (LTQ57_15590)	3372824..3373105	-	282	WP_029248407.1	hypothetical protein	-
LTQ57_RS23280	3373235..3373429	-	195	Protein_3081	helix-turn-helix domain containing protein	-
LTQ57_RS15600 (LTQ57_15600)	3373525..3374262	+	738	WP_009975393.1	SDR family oxidoreductase	-
LTQ57_RS15605 (LTQ57_15605)	3374328..3374768	-	441	WP_003875392.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
LTQ57_RS15610 (LTQ57_15610)	3374868..3375239	-	372	WP_003875391.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTQ57_RS15615 (LTQ57_15615)	3375226..3375432	-	207	WP_011724021.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LTQ57_RS15620 (LTQ57_15620)	3375601..3376545	-	945	WP_003875389.1	HNH endonuclease signature motif containing protein	-
LTQ57_RS15625 (LTQ57_15625)	3376745..3377818	-	1074	WP_009975391.1	redox-regulated ATPase YchF	-
LTQ57_RS15630 (LTQ57_15630)	3377949..3379142	+	1194	WP_080516334.1	hypothetical protein	-
LTQ57_RS15635 (LTQ57_15635)	3379159..3380157	-	999	WP_009975388.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13770.14 Da
• Isoelectric Point: 6.6003

>T227853 WP_003875391.1 NZ_CP089223:c3375239-3374868 [Mycobacterium avium subsp. avium]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVECHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Nucleotide

• Length: 372 bp

>T227853 NZ_CP123197:1539560-1539663 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7261.25 Da
• Isoelectric Point: 11.0634

>AT227853 WP_011724021.1 NZ_CP089223:c3375432-3375226 [Mycobacterium avium subsp. avium]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRTPADDSR

Nucleotide

• Length: 207 bp

>AT227853 NZ_CP123197:c1539701-1539430 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A0E2WB37