Detailed information of TA system TA227850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 5173663..5174316
• Replicon: chromosome
• Accession: NZ_CP089222
• Organism: Mycobacterium intracellulare subsp. intracellulare strain FDAARGOS_1610

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LTQ56_RS24315
• Protein ID: WP_008253911.1
• Coordinates: 5173855..5174316 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X8A187
• Locus tag: LTQ56_RS24310
• Protein ID: WP_009957321.1
• Coordinates: 5173663..5173851 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTQ56_RS24300 (LTQ56_24300)	5169636..5171213	+	1578	WP_009957319.1	serine hydrolase	-
LTQ56_RS24305 (LTQ56_24305)	5171210..5173597	+	2388	WP_009957320.1	cation-translocating P-type ATPase	-
LTQ56_RS24310 (LTQ56_24310)	5173663..5173851	+	189	WP_009957321.1	antitoxin	Antitoxin
LTQ56_RS24315 (LTQ56_24315)	5173855..5174316	+	462	WP_008253911.1	SRPBCC family protein	Toxin
LTQ56_RS24320 (LTQ56_24320)	5174401..5175171	+	771	WP_009957322.1	VOC family protein	-
LTQ56_RS24325 (LTQ56_24325)	5175245..5175679	+	435	WP_008253914.1	hypothetical protein	-
LTQ56_RS24330 (LTQ56_24330)	5175684..5176172	-	489	WP_008253915.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
LTQ56_RS24335 (LTQ56_24335)	5176172..5177698	-	1527	WP_009957325.1	carotenoid oxygenase family protein	-
LTQ56_RS24340 (LTQ56_24340)	5177700..5178920	-	1221	WP_009957327.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16639.20 Da
• Isoelectric Point: 8.5617

>T227850 WP_008253911.1 NZ_CP089222:5173855-5174316 [Mycobacterium intracellulare subsp. intracellulare]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T227850 NZ_CP123197:1318973-1319224 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6698.30 Da
• Isoelectric Point: 4.4702

>AT227850 WP_009957321.1 NZ_CP089222:5173663-5173851 [Mycobacterium intracellulare subsp. intracellulare]
MGFLDKAKDLLSQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT227850 NZ_CP123197:1319221-1319475 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: X8A187