Detailed information of TA system TA227848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 1146770..1147434
• Replicon: chromosome
• Accession: NZ_CP089222
• Organism: Mycobacterium intracellulare subsp. intracellulare strain FDAARGOS_1610

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: J9WD27
• Locus tag: LTQ56_RS05175
• Protein ID: WP_009954498.1
• Coordinates: 1147027..1147434 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LTQ56_RS05170
• Protein ID: WP_009954496.1
• Coordinates: 1146770..1147030 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTQ56_RS05145 (LTQ56_05145)	1143278..1143769	-	492	WP_014379893.1	DUF1707 domain-containing protein	-
LTQ56_RS05150 (LTQ56_05150)	1143839..1144435	+	597	WP_009954492.1	TetR/AcrR family transcriptional regulator	-
LTQ56_RS05155 (LTQ56_05155)	1144440..1145708	-	1269	WP_009954493.1	cytochrome P450	-
LTQ56_RS05160 (LTQ56_05160)	1145869..1146321	+	453	WP_029384640.1	VOC family protein	-
LTQ56_RS05165 (LTQ56_05165)	1146358..1146597	+	240	WP_009954495.1	hypothetical protein	-
LTQ56_RS05170 (LTQ56_05170)	1146770..1147030	+	261	WP_009954496.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LTQ56_RS05175 (LTQ56_05175)	1147027..1147434	+	408	WP_009954498.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTQ56_RS05180 (LTQ56_05180)	1147612..1150713	+	3102	WP_009954499.1	DEAD/DEAH box helicase	-
LTQ56_RS05185 (LTQ56_05185)	1150775..1152340	-	1566	WP_009954500.1	protein kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-66)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14293.28 Da
• Isoelectric Point: 4.2772

>T227848 WP_009954498.1 NZ_CP089222:1147027-1147434 [Mycobacterium intracellulare subsp. intracellulare]
MSNPQVAGVLDTSVFIATESGRPLDAALIPDQVGTTVVTLAELHVGVLAASTSDIRAQRLATLESIADIEALPVDDDAAR
MWARLRIHLAETGQRVRINDLWIAAIAASRGLPVVTQDDDFAVLDGAANLTVIRV

Nucleotide

• Length: 408 bp

>T227848 NZ_CP123197:597350-597661 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9526.85 Da
• Isoelectric Point: 9.1203

>AT227848 WP_009954496.1 NZ_CP089222:1146770-1147030 [Mycobacterium intracellulare subsp. intracellulare]
MSEVASRELRNDTAGVLRRVRDGEDVTITVSGRPVAVLTAVVPQRRRWLSKTELLSRLPRVQADPGLREDLHALAGDTTE
ELGPIR

Nucleotide

• Length: 261 bp

>AT227848 NZ_CP123197:597658-598077 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: J9WD27