Detailed information of TA system TA227847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 11980..12544
• Replicon: chromosome
• Accession: NZ_CP089222
• Organism: Mycobacterium intracellulare subsp. intracellulare strain FDAARGOS_1610

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H8IVI4
• Locus tag: LTQ56_RS00060
• Protein ID: WP_009953437.1
• Coordinates: 12173..12544 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LTQ56_RS00055
• Protein ID: WP_026071432.1
• Coordinates: 11980..12186 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTQ56_RS00035 (LTQ56_00035)	7601..8599	+	999	WP_008254378.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LTQ56_RS00040 (LTQ56_00040)	8645..9784	-	1140	WP_179300624.1	hypothetical protein	-
LTQ56_RS00045 (LTQ56_00045)	9926..10999	+	1074	WP_009953440.1	redox-regulated ATPase YchF	-
LTQ56_RS00050 (LTQ56_00050)	11079..11741	-	663	WP_014379386.1	methyltransferase domain-containing protein	-
LTQ56_RS00055 (LTQ56_00055)	11980..12186	+	207	WP_026071432.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LTQ56_RS00060 (LTQ56_00060)	12173..12544	+	372	WP_009953437.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTQ56_RS00065 (LTQ56_00065)	12602..13153	+	552	WP_009953436.1	VOC family protein	-
LTQ56_RS00070 (LTQ56_00070)	13232..13630	+	399	WP_009953435.1	VOC family protein	-
LTQ56_RS00075 (LTQ56_00075)	13729..14112	+	384	WP_026071433.1	hypothetical protein	-
LTQ56_RS00080 (LTQ56_00080)	14198..14557	+	360	WP_198139476.1	hypothetical protein	-
LTQ56_RS00085 (LTQ56_00085)	14682..15110	+	429	Protein_16	hypothetical protein	-
LTQ56_RS00090 (LTQ56_00090)	15677..16000	+	324	WP_009953431.1	putative quinol monooxygenase	-
LTQ56_RS00095 (LTQ56_00095)	16049..16732	-	684	WP_014384004.1	hypothetical protein	-
LTQ56_RS00100 (LTQ56_00100)	16879..17088	+	210	WP_014381976.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13868.20 Da
• Isoelectric Point: 6.8398

>T227847 WP_009953437.1 NZ_CP089222:12173-12544 [Mycobacterium intracellulare subsp. intracellulare]
VILVDTSIWIDHLHVAEQRLVGRLTNDEIGCHQMVIEELALGMIKQRDDVLRLLSHLREFPLLTHAEILHLVDRRRLWGR
GLSAIDAHLLGSVAVVDGARLWTRDKKLKAACKEVGVAIVDES

Nucleotide

• Length: 372 bp

>T227847 NZ_CP123197:531100-531435 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7250.31 Da
• Isoelectric Point: 11.9169

>AT227847 WP_026071432.1 NZ_CP089222:11980-12186 [Mycobacterium intracellulare subsp. intracellulare]
MRTTVTIDDALLAQAAELTGVTESAALLRQGLQTLIRVESARRLAALGGTDPKASAAPRRRPPTRDPR

Nucleotide

• Length: 207 bp

>AT227847 NZ_CP123197:530852-531100 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7R7MQX9