Detailed information of TA system TA227846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 5443992..5444645
• Replicon: chromosome
• Accession: NZ_CP089221
• Organism: Mycobacterium intracellulare strain FDAARGOS_1611

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LTS63_RS25565
• Protein ID: WP_008253911.1
• Coordinates: 5444184..5444645 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: I2A997
• Locus tag: LTS63_RS25560
• Protein ID: WP_008253910.1
• Coordinates: 5443992..5444180 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTS63_RS25550 (LTS63_25550)	5439965..5441542	+	1578	WP_233042837.1	serine hydrolase	-
LTS63_RS25555 (LTS63_25555)	5441539..5443926	+	2388	WP_233042401.1	cation-translocating P-type ATPase	-
LTS63_RS25560 (LTS63_25560)	5443992..5444180	+	189	WP_008253910.1	antitoxin	Antitoxin
LTS63_RS25565 (LTS63_25565)	5444184..5444645	+	462	WP_008253911.1	SRPBCC family protein	Toxin
LTS63_RS25570 (LTS63_25570)	5444730..5445500	+	771	WP_009957322.1	VOC family protein	-
LTS63_RS25575 (LTS63_25575)	5445574..5446008	+	435	WP_008253914.1	hypothetical protein	-
LTS63_RS25580 (LTS63_25580)	5446013..5446501	-	489	WP_008253915.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
LTS63_RS25585 (LTS63_25585)	5446501..5448027	-	1527	WP_014381862.1	carotenoid oxygenase family protein	-
LTS63_RS25590 (LTS63_25590)	5448029..5449249	-	1221	WP_009957327.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16639.20 Da
• Isoelectric Point: 8.5617

>T227846 WP_008253911.1 NZ_CP089221:5444184-5444645 [Mycobacterium intracellulare]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T227846 NZ_CP123197:389508-389915 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6797.44 Da
• Isoelectric Point: 4.4702

>AT227846 WP_008253910.1 NZ_CP089221:5443992-5444180 [Mycobacterium intracellulare]
MGFLDKAKDLLWQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT227846 NZ_CP123197:389261-389527 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7I7TPT2