Detailed information of TA system TA227845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 1751371..1752363
• Replicon: chromosome
• Accession: NZ_CP089221
• Organism: Mycobacterium intracellulare strain FDAARGOS_1611

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: LTS63_RS07835
• Protein ID: WP_038536181.1
• Coordinates: 1751785..1752363 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: X8A634
• Locus tag: LTS63_RS07830
• Protein ID: WP_038536179.1
• Coordinates: 1751371..1751760 (+)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTS63_RS07805 (LTS63_07805)	1746822..1748825	+	2004	WP_095562692.1	phosphotransferase	-
LTS63_RS07810 (LTS63_07810)	1748822..1749322	+	501	WP_038536171.1	gluconokinase	-
LTS63_RS07815 (LTS63_07815)	1749393..1750004	-	612	WP_095562693.1	TetR/AcrR family transcriptional regulator	-
LTS63_RS07820 (LTS63_07820)	1750040..1750999	+	960	WP_225331100.1	SAM-dependent methyltransferase	-
LTS63_RS07825 (LTS63_07825)	1751027..1751314	+	288	WP_014941865.1	hypothetical protein	-
LTS63_RS07830 (LTS63_07830)	1751371..1751760	+	390	WP_038536179.1	hypothetical protein	Antitoxin
LTS63_RS07835 (LTS63_07835)	1751785..1752363	+	579	WP_038536181.1	zeta toxin family protein	Toxin
LTS63_RS07840 (LTS63_07840)	1752383..1753450	-	1068	WP_038536184.1	DoxX family protein	-
LTS63_RS07845 (LTS63_07845)	1753546..1754106	+	561	WP_042911761.1	pyrazinamidase PncA	-
LTS63_RS07850 (LTS63_07850)	1754106..1754906	+	801	WP_009956375.1	nuclear transport factor 2 family protein	-
LTS63_RS07855 (LTS63_07855)	1754918..1755268	-	351	WP_020188920.1	DoxX family protein	-
LTS63_RS07860 (LTS63_07860)	1755434..1756405	+	972	WP_014382494.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-137)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21103.22 Da
• Isoelectric Point: 11.0233

>T227845 WP_038536181.1 NZ_CP089221:1751785-1752363 [Mycobacterium intracellulare]
MQRLDLVVGPNGAGKSTFIPFTLAPLLPGSPVVNADEIARQRWPGESAGRAYDAAQIAAATRAKLIDLGRSFIAETVFSH
PSKLDLIRTAHRAGFTVVLHAMLIPEDLAVERVRHRVRAGGHDVPETKVRERHRRLWTPVTDAIALSDVATVYDNSRLRG
PRIVARLSGGMTIGSPDWPAWAPEILRSRWPD

Nucleotide

• Length: 579 bp

>T227845 NZ_CP123197:255391-255687 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATACCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13507.88 Da
• Isoelectric Point: 5.5186

>AT227845 WP_038536179.1 NZ_CP089221:1751371-1751760 [Mycobacterium intracellulare]
MADAPDRVTRVASDLMDSAASEGARQSRSAKQQLDHWARVGRAVSSQHTASRRRVEAALAGHLATGELTVEEGVVFNAEI
SAAIDESLVHADYGATLAGHGVTTVALNEDGDIVEHRPDGAAVVLARGR

Nucleotide

• Length: 390 bp

>AT227845 NZ_CP123197:255689-256084 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: X8A634