Detailed information of TA system TA227844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 192925..193489
• Replicon: chromosome
• Accession: NZ_CP089221
• Organism: Mycobacterium intracellulare strain FDAARGOS_1611

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LTS63_RS00895
• Protein ID: WP_036458315.1
• Coordinates: 193118..193489 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LTS63_RS00890
• Protein ID: WP_036458377.1
• Coordinates: 192925..193131 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTS63_RS00870 (LTS63_00870)	188540..189538	+	999	WP_233042431.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LTS63_RS00875 (LTS63_00875)	189584..190729	-	1146	WP_196233638.1	hypothetical protein	-
LTS63_RS00880 (LTS63_00880)	190871..191944	+	1074	WP_036458307.1	redox-regulated ATPase YchF	-
LTS63_RS00885 (LTS63_00885)	192024..192686	-	663	WP_036458309.1	methyltransferase domain-containing protein	-
LTS63_RS00890 (LTS63_00890)	192925..193131	+	207	WP_036458377.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LTS63_RS00895 (LTS63_00895)	193118..193489	+	372	WP_036458315.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTS63_RS00900 (LTS63_00900)	193547..194098	+	552	WP_038535279.1	VOC family protein	-
LTS63_RS00905 (LTS63_00905)	194177..194575	+	399	WP_009953435.1	VOC family protein	-
LTS63_RS00910 (LTS63_00910)	194675..195058	+	384	WP_196233639.1	hypothetical protein	-
LTS63_RS00915 (LTS63_00915)	195132..195476	-	345	WP_225346283.1	hypothetical protein	-
LTS63_RS00920 (LTS63_00920)	195628..196056	+	429	Protein_180	hypothetical protein	-
LTS63_RS00925 (LTS63_00925)	196623..196946	+	324	WP_009953431.1	putative quinol monooxygenase	-
LTS63_RS00930 (LTS63_00930)	196995..197678	-	684	WP_014384004.1	hypothetical protein	-
LTS63_RS00935 (LTS63_00935)	197825..198034	+	210	WP_014381976.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13905.22 Da
• Isoelectric Point: 6.9118

>T227844 WP_036458315.1 NZ_CP089221:193118-193489 [Mycobacterium intracellulare]
VILVDTSIWIDHLHVAEHRLVGRLTNDEIGCHQMVIEELALGMIKQRDDVLRLLSHLREFPLLTHAEILHLVDRRRLWGR
GLSAIDAHLLGSVAVVDGARLWTRDKKLKAACREVGVAIVDES

Nucleotide

• Length: 372 bp

>T227844 NZ_CP123197:c142316-141852 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7220.29 Da
• Isoelectric Point: 11.9169

>AT227844 WP_036458377.1 NZ_CP089221:192925-193131 [Mycobacterium intracellulare]
MRTTVTIDDALLAQAAELTGVAESAALLRQGLQTLIRVESARRLAALGGTDPKASAAPRRRPPTRDPR

Nucleotide

• Length: 207 bp

>AT227844 NZ_CP123197:c142651-142316 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA