Detailed information of TA system TA227840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 575188..575752
• Replicon: chromosome
• Accession: NZ_CP089220
• Organism: Mycobacterium intracellulare strain FDAARGOS_1612

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H8IVI4
• Locus tag: LTQ55_RS02860
• Protein ID: WP_009953437.1
• Coordinates: 575381..575752 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LTQ55_RS02855
• Protein ID: WP_026071432.1
• Coordinates: 575188..575394 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTQ55_RS02835 (LTQ55_02835)	570809..571807	+	999	WP_008254378.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LTQ55_RS02840 (LTQ55_02840)	571853..572992	-	1140	WP_179300624.1	hypothetical protein	-
LTQ55_RS02845 (LTQ55_02845)	573134..574207	+	1074	WP_009953440.1	redox-regulated ATPase YchF	-
LTQ55_RS02850 (LTQ55_02850)	574287..574949	-	663	WP_014379386.1	methyltransferase domain-containing protein	-
LTQ55_RS02855 (LTQ55_02855)	575188..575394	+	207	WP_026071432.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LTQ55_RS02860 (LTQ55_02860)	575381..575752	+	372	WP_009953437.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTQ55_RS02865 (LTQ55_02865)	575810..576361	+	552	WP_009953436.1	VOC family protein	-
LTQ55_RS02870 (LTQ55_02870)	576440..576838	+	399	WP_009953435.1	VOC family protein	-
LTQ55_RS02875 (LTQ55_02875)	576937..577320	+	384	WP_026071433.1	hypothetical protein	-
LTQ55_RS02880 (LTQ55_02880)	577406..577765	+	360	WP_198139476.1	hypothetical protein	-
LTQ55_RS02885 (LTQ55_02885)	577890..578318	+	429	Protein_567	hypothetical protein	-
LTQ55_RS02890 (LTQ55_02890)	578885..579208	+	324	WP_009953431.1	putative quinol monooxygenase	-
LTQ55_RS02895 (LTQ55_02895)	579257..579940	-	684	WP_014384004.1	hypothetical protein	-
LTQ55_RS02900 (LTQ55_02900)	580087..580296	+	210	WP_014381976.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13868.20 Da
• Isoelectric Point: 6.8398

>T227840 WP_009953437.1 NZ_CP089220:575381-575752 [Mycobacterium intracellulare]
VILVDTSIWIDHLHVAEQRLVGRLTNDEIGCHQMVIEELALGMIKQRDDVLRLLSHLREFPLLTHAEILHLVDRRRLWGR
GLSAIDAHLLGSVAVVDGARLWTRDKKLKAACKEVGVAIVDES

Nucleotide

• Length: 372 bp

>T227840 NZ_CP123195:c11629-11231 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7250.31 Da
• Isoelectric Point: 11.9169

>AT227840 WP_026071432.1 NZ_CP089220:575188-575394 [Mycobacterium intracellulare]
MRTTVTIDDALLAQAAELTGVTESAALLRQGLQTLIRVESARRLAALGGTDPKASAAPRRRPPTRDPR

Nucleotide

• Length: 207 bp

>AT227840 NZ_CP123195:c11856-11629 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A7R7MQX9