Detailed information of TA system TA227839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 323332..323985
• Replicon: chromosome
• Accession: NZ_CP089220
• Organism: Mycobacterium intracellulare strain FDAARGOS_1612

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LTQ55_RS01695
• Protein ID: WP_008253911.1
• Coordinates: 323524..323985 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X8A187
• Locus tag: LTQ55_RS01690
• Protein ID: WP_009957321.1
• Coordinates: 323332..323520 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTQ55_RS01680 (LTQ55_01680)	319305..320882	+	1578	WP_009957319.1	serine hydrolase	-
LTQ55_RS01685 (LTQ55_01685)	320879..323266	+	2388	WP_009957320.1	cation-translocating P-type ATPase	-
LTQ55_RS01690 (LTQ55_01690)	323332..323520	+	189	WP_009957321.1	antitoxin	Antitoxin
LTQ55_RS01695 (LTQ55_01695)	323524..323985	+	462	WP_008253911.1	SRPBCC family protein	Toxin
LTQ55_RS01700 (LTQ55_01700)	324070..324840	+	771	WP_009957322.1	VOC family protein	-
LTQ55_RS01705 (LTQ55_01705)	324914..325348	+	435	WP_008253914.1	hypothetical protein	-
LTQ55_RS01710 (LTQ55_01710)	325353..325841	-	489	WP_008253915.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
LTQ55_RS01715 (LTQ55_01715)	325841..327367	-	1527	WP_014381862.1	carotenoid oxygenase family protein	-
LTQ55_RS01720 (LTQ55_01720)	327369..328589	-	1221	WP_009957327.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16639.20 Da
• Isoelectric Point: 8.5617

>T227839 WP_008253911.1 NZ_CP089220:323524-323985 [Mycobacterium intracellulare]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T227839 NZ_CP123057:1603399-1603686 [Brucella intermedia]
ATGCCTTATAAGCTTGAATTCCTGCCTTCAGCGCTTAAAGAATGGAATAAACTTGGCTCAACAATCCGAGAGCAATTAAA
GAAAAAATTGCGTGAACGTTTGGAAACGCCGCGGGTCGTTAGCGCCTCACTTTACGGTATGCCTGACCATTACAAGATAA
AATTACGTCAATTAGGCTATCGGCTGGTTTATTCCGTCAACGACGACACCGTGACGGTGTTGGTCGTGGCAGTTGGAAGA
CGTGAGCGTGGCGATGTTTACAATACCGCTCGCACCAGAACACAGTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6698.30 Da
• Isoelectric Point: 4.4702

>AT227839 WP_009957321.1 NZ_CP089220:323332-323520 [Mycobacterium intracellulare]
MGFLDKAKDLLSQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT227839 NZ_CP123057:1603164-1603409 [Brucella intermedia]
ATGCAACGTGTGGAAGCAAATATAGCAGTTAGTGTATCGGACCTCAAAAAAAGCCCCTCCGCGGTAATGGACGAGGCTAA
GGGCGAGGCTGTCGCCGTTCTTAATCATAATCGAATTATGGCTTATATGGTTCCCGCAGATGTTTATGAAGCCATGCTCG
AGCAGTTAGACGATATTCGCCTTACTGAGATAATCCGGAAACGAGCCGACGAAAAAGGAATTTCGGTCGATATCGATGCC
TTATAA

Structures

Antitoxin

• AlphaFold DB: X8A187