Detailed information of TA system TA227838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 4726586..4727207
• Replicon: chromosome
• Accession: NZ_CP089218
• Organism: Mycobacterium kansasii strain FDAARGOS_1614

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LTT71_RS20495
• Protein ID: WP_023367403.1
• Coordinates: 4726586..4727023 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X7ZGA3
• Locus tag: LTT71_RS20500
• Protein ID: WP_023367405.1
• Coordinates: 4727028..4727207 (-)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTT71_RS20470 (LTT71_20470)	4722379..4723893	+	1515	WP_036393600.1	carotenoid oxygenase family protein	-
LTT71_RS20475 (LTT71_20475)	4723973..4724455	+	483	WP_023367395.1	VOC family protein	-
LTT71_RS20480 (LTT71_20480)	4724461..4725198	-	738	WP_023367397.1	trimeric intracellular cation channel family protein	-
LTT71_RS20485 (LTT71_20485)	4725240..4725659	-	420	WP_023367399.1	hypothetical protein	-
LTT71_RS20490 (LTT71_20490)	4725696..4726478	-	783	WP_036393899.1	VOC family protein	-
LTT71_RS20495 (LTT71_20495)	4726586..4727023	-	438	WP_023367403.1	SRPBCC family protein	Toxin
LTT71_RS20500 (LTT71_20500)	4727028..4727207	-	180	WP_023367405.1	antitoxin	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-141)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15888.48 Da
• Isoelectric Point: 8.5679

>T227838 WP_023367403.1 NZ_CP089218:c4727023-4726586 [Mycobacterium kansasii]
MAKLSGSIDVPLPPEEAWLHASNLSRYREWLTIHRVWRSKLPETLERGAVVESIVEVKGMPNRIRWTIVRYKPPEGMTLN
GDGVGGVKVKLMAKVAPKEEGSVVSFDVHLGGPALFGPIGMIVAAALRSDISESLQNFVEVFAQA

Nucleotide

• Length: 438 bp

>T227838 NZ_CP123056:c279353-279063 [Brucella intermedia]
ATGATCGAAGTCCGCCAAACCACCTTTTTCACCAAGTGGCTTGATGAGCTCCGTGATACCAATGCACGGTTGCGGATTGT
CACTCGGATCCGCCGCATGGAACTGGGCAATCCCGGCGATGTGAAATCTGTCGGCGAAGGCGTGAGCGAAATGCGCATTA
CGTACGGCCCCGGCTATCGGGTTTATTTCGTATCCATGGGATCAACTATCGTTGTTCTGCTGTGTGGTGGCGACAAGTCA
TCACAGAGCCGGGATATCGCGCTGGCGAAACAAATGGCGAAGGAGATTTAG

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6552.35 Da
• Isoelectric Point: 5.7603

>AT227838 WP_023367405.1 NZ_CP089218:c4727207-4727028 [Mycobacterium kansasii]
MGIMDKAKDLLSQNADKVETVIDKAGKFVDDKTQGKYSDTIHKVQEQAKKVVDTGDQQN

Nucleotide

• Length: 180 bp

>AT227838 NZ_CP123056:c279061-278762 [Brucella intermedia]
ATGGTCCTTGAAACCACCCGTTTTGATGTTCTTGATCACCTCAAGACGCCGGAAGAGCGTGTTGCCTATCTGGAAGCGGC
ATTTGAAGATGGCGACCCTTCCCTTATTGCGCATGCTCTTGGTGACGTAGCCCGTTCCATTGGCATGACTACTGTCGCTA
AAGAAGCCGGAATTACCCGTGAAGCCCTTTACCGCTCCCTGAGCGAGAACGGCGATCCAAAACTATCGACCTTGCTCGGC
GTCATGAAGGCCCTTGGCCTGCACCTGTCTGTCACAGCCACGGATAAACGCGCCGCTTGA

Structures

Antitoxin

• AlphaFold DB: X7ZGA3