Detailed information of TA system TA227836

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 3861107..3861799
• Replicon: chromosome
• Accession: NZ_CP089218
• Organism: Mycobacterium kansasii strain FDAARGOS_1614

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7ZNI0
• Locus tag: LTT71_RS16760
• Protein ID: WP_023365904.1
• Coordinates: 3861377..3861799 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: X7ZK45
• Locus tag: LTT71_RS16755
• Protein ID: WP_036392951.1
• Coordinates: 3861107..3861370 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTT71_RS16720 (LTT71_16720)	3856179..3857681	-	1503	WP_023365894.1	trigger factor	-
LTT71_RS16735 (LTT71_16735)	3858042..3859226	+	1185	WP_023365896.1	serine hydrolase domain-containing protein	-
LTT71_RS16740 (LTT71_16740)	3859273..3859599	-	327	WP_133163539.1	hypothetical protein	-
LTT71_RS16745 (LTT71_16745)	3859687..3860493	-	807	WP_023365898.1	zinc finger domain-containing protein	-
LTT71_RS16750 (LTT71_16750)	3860527..3861006	-	480	WP_023365900.1	ribose-5-phosphate isomerase	-
LTT71_RS16755 (LTT71_16755)	3861107..3861370	+	264	WP_036392951.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LTT71_RS16760 (LTT71_16760)	3861377..3861799	+	423	WP_023365904.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTT71_RS16765 (LTT71_16765)	3861796..3862407	-	612	WP_023365906.1	Rv2466c family mycothiol-dependent reductase	-
LTT71_RS16770 (LTT71_16770)	3862548..3865151	+	2604	WP_036392948.1	aminopeptidase N	-
LTT71_RS16775 (LTT71_16775)	3865219..3865686	-	468	WP_036392579.1	DUF5130 domain-containing protein	-
LTT71_RS16780 (LTT71_16780)	3865673..3865900	-	228	WP_023365912.1	hypothetical protein	-
LTT71_RS16785 (LTT71_16785)	3865963..3866637	-	675	WP_036392577.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-83)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15092.17 Da
• Isoelectric Point: 5.0719

>T227836 WP_023365904.1 NZ_CP089218:3861377-3861799 [Mycobacterium kansasii]
VILDSSAFVALIQGEAPYTEQIAAALTGDRSPVMSTANAAECLIVLTSRHGFIARTVFDRLRFEINLGFQPFNFELAMAA
HLAYLRYGRGRHPAGLNCGDTMAYATAKLAHEPLIAIGNDFAQTDLESDGVIGYWPSPCG

Nucleotide

• Length: 423 bp

>T227836 NZ_CP123050:c400737-400438 [Bifidobacterium adolescentis]
ATGGGCCGGTTGACCGCCGACTTCGCGAAGGCCTTCAGCCGCGATCTGAAAAAGAACGCCAAACGGCGTAACTGGAACCT
GACCGAGCTTGAAAAAGTCATCGATCTGGTAGTGGAGAACACCCCGGAAACGCTGGAGGAGCTGCGCCGCCGCCACAACA
TGCACACCCTGTCCGGCAATTGGCGCGGGCGATACGAATGCCATGTCGCCAATGCCGGGGACTGGCTAGTGATCTGGTCA
TCGAACGATTCAGTCGCATTCTTCGAACGCACCGGCAGCCACGACGAATTATTCCGGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9890.16 Da
• Isoelectric Point: 5.6277

>AT227836 WP_036392951.1 NZ_CP089218:3861107-3861370 [Mycobacterium kansasii]
VAFNVKDEEVIRLADELAARLHHPSRIDAIRYALRAQIEITQSRTANRADELLDVLRTEIWPLLHDRSPITKSEREQALG
YDAATGV

Nucleotide

• Length: 264 bp

>AT227836 NZ_CP123050:c401009-400737 [Bifidobacterium adolescentis]
ATGAGTGCATCAACGTTGACCATACGGATGGATGACGAACTCAAGCGTTCGGCCGCCGACGTGGCCGAATATTATGGCTT
GGATCTCTCGTCAGTGGTGCGCGCCGTATTCAAGCAAATGGTGAATACCGGGCGCATCCCATTGACGTTCGCCCCCGAGG
AGCCTAATGGCGAAAGCCTGAACGCCGCTGCCGAAGCGGACAGCTTCATGGCATCGGGCAAGACGGGGCGTTTTACCAAC
GGACACGACCTGCTCGAAGCGGCGATGCGCTGA

Structures

Toxin

• AlphaFold DB: X7ZNI0

Antitoxin

• AlphaFold DB: X7ZK45