Detailed information of TA system TA227835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 3717686..3718263
• Replicon: chromosome
• Accession: NZ_CP089218
• Organism: Mycobacterium kansasii strain FDAARGOS_1614

Toxin (Protein)

• Gene name: doc
• Uniprot ID: X7ZN33
• Locus tag: LTT71_RS16150
• Protein ID: WP_023365653.1
• Coordinates: 3717880..3718263 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: X7ZMP8
• Locus tag: LTT71_RS16145
• Protein ID: WP_023365651.1
• Coordinates: 3717686..3717883 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTT71_RS16120 (LTT71_16120)	3712830..3713366	-	537	WP_023365641.1	rRNA maturation RNase YbeY	-
LTT71_RS16125 (LTT71_16125)	3713416..3714465	-	1050	WP_036392639.1	PhoH family protein	-
LTT71_RS16130 (LTT71_16130)	3714612..3716057	-	1446	WP_133163534.1	cytochrome P450	-
LTT71_RS16135 (LTT71_16135)	3716140..3716931	-	792	WP_023365647.1	class I SAM-dependent methyltransferase	-
LTT71_RS16140 (LTT71_16140)	3717001..3717570	-	570	WP_023365649.1	carboxymethylenebutenolidase	-
LTT71_RS16145 (LTT71_16145)	3717686..3717883	+	198	WP_023365651.1	hypothetical protein	Antitoxin
LTT71_RS16150 (LTT71_16150)	3717880..3718263	+	384	WP_023365653.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LTT71_RS16155 (LTT71_16155)	3718286..3719032	-	747	WP_023365655.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
LTT71_RS16160 (LTT71_16160)	3719051..3720199	-	1149	WP_023365657.1	molecular chaperone DnaJ	-
LTT71_RS16165 (LTT71_16165)	3720259..3721290	-	1032	WP_023365659.1	heat-inducible transcriptional repressor HrcA	-
LTT71_RS16170 (LTT71_16170)	3721506..3721820	+	315	WP_023365661.1	type II toxin-antitoxin system VapB family antitoxin	-
LTT71_RS16175 (LTT71_16175)	3721978..3722327	+	350	Protein_3211	hypothetical protein	-
LTT71_RS16180 (LTT71_16180)	3722359..3722938	+	580	Protein_3212	sigma factor-like helix-turn-helix DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (19-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13726.39 Da
• Isoelectric Point: 3.9949

>T227835 WP_023365653.1 NZ_CP089218:3717880-3718263 [Mycobacterium kansasii]
VTEFFDRDDVLTAGALAVGAVLEVGDFGLLDAAVARPQATVYGVDAYPDLYTKAAALLQSLARNRALVDGNKRTAWASAW
TFLYINGIELVAGFDVDTAERFMYDVAERGELTVDDIAETLRQFASA

Nucleotide

• Length: 384 bp

>T227835 NZ_CP123048:79519-79824 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGAGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6940.89 Da
• Isoelectric Point: 11.0918

>AT227835 WP_023365651.1 NZ_CP089218:3717686-3717883 [Mycobacterium kansasii]
VPSLNIGFTDEELVAVRDAAAGENLSLRAFVHRAAVVAASDRKRRVAEAAALVAQRSAELNRRLA

Nucleotide

• Length: 198 bp

>AT227835 NZ_CP123048:79299-79517 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: X7ZN33

Antitoxin

• AlphaFold DB: X7ZMP8