Detailed information of TA system TA227833

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 1997994..1998679
• Replicon: chromosome
• Accession: NZ_CP089218
• Organism: Mycobacterium kansasii strain FDAARGOS_1614

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7ZUB0
• Locus tag: LTT71_RS08815
• Protein ID: WP_023373049.1
• Coordinates: 1998269..1998679 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LTT71_RS08810
• Protein ID: WP_036392171.1
• Coordinates: 1997994..1998272 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTT71_RS08790 (LTT71_08790)	1994937..1995449	+	513	WP_036391447.1	Holliday junction resolvase RuvX	-
LTT71_RS08795 (LTT71_08795)	1995442..1996689	+	1248	WP_023373040.1	endolytic transglycosylase MltG	-
LTT71_RS08800 (LTT71_08800)	1996667..1997491	+	825	WP_023373043.1	shikimate dehydrogenase	-
LTT71_RS08805 (LTT71_08805)	1997503..1997931	+	429	WP_023373045.1	A24 family peptidase	-
LTT71_RS08810 (LTT71_08810)	1997994..1998272	+	279	WP_036392171.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LTT71_RS08815 (LTT71_08815)	1998269..1998679	+	411	WP_023373049.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTT71_RS08820 (LTT71_08820)	1998808..2000013	+	1206	WP_023373051.1	chorismate synthase	-
LTT71_RS08825 (LTT71_08825)	2000030..2000566	+	537	WP_023373053.1	shikimate kinase	-
LTT71_RS08830 (LTT71_08830)	2000563..2001651	+	1089	WP_023373055.1	3-dehydroquinate synthase	-
LTT71_RS08835 (LTT71_08835)	2001648..2002088	+	441	WP_023373057.1	type II 3-dehydroquinate dehydratase	-
LTT71_RS08840 (LTT71_08840)	2002085..2002837	-	753	WP_036448656.1	B-4DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-48)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14705.90 Da
• Isoelectric Point: 4.8944

>T227833 WP_023373049.1 NZ_CP089218:1998269-1998679 [Mycobacterium kansasii]
VIYLDTSALTKLLIAEPETDELRNWLTVHTDQGEFAATSALSRVELMRVVARQGESGQAERARYLLDGLDILPLTAPVIN
LAETIGPATLRTLDAIHLAAATQIKRELTAFVTYDHRLLAGCLEVGLVTASPGAVR

Nucleotide

• Length: 411 bp

>T227833 NZ_CP123047:97088-97375 [Escherichia coli]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10114.45 Da
• Isoelectric Point: 4.8604

>AT227833 WP_036392171.1 NZ_CP089218:1997994-1998272 [Mycobacterium kansasii]
VEAIGVRELRQHASRYLARVEAGEELGITNNGRLVARLVPVQAAERSREALIESGTLIPAPCPQNLLDVTATPAPDRKRT
LSDVLDEMRDEQ

Nucleotide

• Length: 279 bp

>AT227833 NZ_CP123047:96840-97091 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: X7ZUB0