Detailed information of TA system TA227830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 404897..405522
• Replicon: chromosome
• Accession: NZ_CP089218
• Organism: Mycobacterium kansasii strain FDAARGOS_1614

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7YX14
• Locus tag: LTT71_RS01795
• Protein ID: WP_023370764.1
• Coordinates: 405121..405522 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: X7YXT3
• Locus tag: LTT71_RS01790
• Protein ID: WP_023370762.1
• Coordinates: 404897..405124 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTT71_RS01775 (LTT71_01775)	400192..401358	+	1167	WP_023370756.1	LarC family nickel insertion protein	-
LTT71_RS01780 (LTT71_01780)	401365..402537	-	1173	WP_023370758.1	MFS transporter	-
LTT71_RS01785 (LTT71_01785)	402833..404776	-	1944	Protein_356	SEC-C domain-containing protein	-
LTT71_RS01790 (LTT71_01790)	404897..405124	+	228	WP_023370762.1	hypothetical protein	Antitoxin
LTT71_RS01795 (LTT71_01795)	405121..405522	+	402	WP_023370764.1	PIN domain-containing protein	Toxin
LTT71_RS01800 (LTT71_01800)	405607..406563	+	957	WP_023370766.1	class I SAM-dependent methyltransferase	-
LTT71_RS01805 (LTT71_01805)	406743..407867	+	1125	WP_230589192.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-120)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14053.99 Da
• Isoelectric Point: 6.3696

>T227830 WP_023370764.1 NZ_CP089218:405121-405522 [Mycobacterium kansasii]
VIALLDSNVLIALVVAEHVHHDVAAAWLSARDNGFATCPITQGSLVRFLMRTGQPAAVAREVVGAVETAKRHEFWPDSVS
FADVEIGGVVGHRQVTDAYLAQLARSRNGQLATLDSGLAHLHSDVAVLIPTAG

Nucleotide

• Length: 402 bp

>T227830 NZ_CP123047:25606-25911 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8012.00 Da
• Isoelectric Point: 4.7377

>AT227830 WP_023370762.1 NZ_CP089218:404897-405124 [Mycobacterium kansasii]
VRTTIDLPDDLHKQALAIARDTRRTLSETVADLMRRGLGAGGAAAISTDPRTGLPLVNVGKVVTSEDVRSLEDEE

Nucleotide

• Length: 228 bp

>AT227830 NZ_CP123047:25386-25604 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: X7YX14

Antitoxin

• AlphaFold DB: X7YXT3