Detailed information of TA system TA227829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 145321..146009
• Replicon: chromosome
• Accession: NZ_CP089218
• Organism: Mycobacterium kansasii strain FDAARGOS_1614

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7Z9W3
• Locus tag: LTT71_RS00625
• Protein ID: WP_023370286.1
• Coordinates: 145321..145752 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LTT71_RS00630
• Protein ID: WP_036394775.1
• Coordinates: 145749..146009 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTT71_RS00590 (LTT71_00590)	140851..141159	+	309	WP_023370272.1	DUF3263 domain-containing protein	-
LTT71_RS00595 (LTT71_00595)	141224..141694	+	471	WP_036394685.1	LytR C-terminal domain-containing protein	-
LTT71_RS00600 (LTT71_00600)	141813..142529	+	717	WP_023370276.1	superoxide dismutase family protein	-
LTT71_RS00605 (LTT71_00605)	142510..143673	+	1164	WP_103797770.1	glutamate--cysteine ligase	-
LTT71_RS00610 (LTT71_00610)	143756..144157	+	402	WP_103797769.1	hypothetical protein	-
LTT71_RS00615 (LTT71_00615)	144229..144468	+	240	WP_155254706.1	hypothetical protein	-
LTT71_RS00620 (LTT71_00620)	144665..145312	+	648	WP_023370284.1	LON peptidase substrate-binding domain-containing protein	-
LTT71_RS00625 (LTT71_00625)	145321..145752	-	432	WP_023370286.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTT71_RS00630 (LTT71_00630)	145749..146009	-	261	WP_036394775.1	CopG family transcriptional regulator	Antitoxin
LTT71_RS00635 (LTT71_00635)	146054..148258	-	2205	WP_023370290.1	AAA family ATPase	-
LTT71_RS00640 (LTT71_00640)	148255..149115	-	861	WP_023370292.1	CDP-diacylglycerol--serine O-phosphatidyltransferase	-
LTT71_RS00645 (LTT71_00645)	149112..149807	-	696	WP_023370294.1	phosphatidylserine decarboxylase	-
LTT71_RS00650 (LTT71_00650)	149928..150177	-	250	Protein_131	molybdopterin molybdenumtransferase MoeA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-134)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-40)

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15954.13 Da
• Isoelectric Point: 5.0701

>T227829 WP_023370286.1 NZ_CP089218:c145752-145321 [Mycobacterium kansasii]
VIIPDVNLLLYAVITGFPQHRRAYAWWHDTVNSQERIGLTYPAVFGFLRIVTNARILTEPLSATEAVAYVRDWLTQPNVD
LLIPSARHLDLALGLLEQLGTASNLTTDVQLAAYAIEHNAEMHSSDADFARFAGLAWTNPLDR

Nucleotide

• Length: 432 bp

>T227829 NZ_CP123047:c21196-20780 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10093.61 Da
• Isoelectric Point: 9.9864

>AT227829 WP_036394775.1 NZ_CP089218:c146009-145749 [Mycobacterium kansasii]
MRTTLTLDDDVVRLIEEAVHRERRPMKHVINDALRKALAQPMERRTPYQLKPHRSAVRPGFDFAGFNRLADEMEDQAILD
QKRPTL

Nucleotide

• Length: 261 bp

>AT227829 NZ_CP123047:c21423-21193 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: X7Z9W3