Detailed information of TA system TA227828

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 6187449..6188070
• Replicon: chromosome
• Accession: NZ_CP089216
• Organism: Mycobacterium kansasii strain FDAARGOS_1615

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LTS70_RS27110
• Protein ID: WP_023367403.1
• Coordinates: 6187449..6187886 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X7ZGA3
• Locus tag: LTS70_RS27115
• Protein ID: WP_023367405.1
• Coordinates: 6187891..6188070 (-)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTS70_RS27085 (LTS70_27085)	6183242..6184756	+	1515	WP_036393600.1	carotenoid oxygenase family protein	-
LTS70_RS27090 (LTS70_27090)	6184836..6185318	+	483	WP_023367395.1	VOC family protein	-
LTS70_RS27095 (LTS70_27095)	6185324..6186061	-	738	WP_023367397.1	trimeric intracellular cation channel family protein	-
LTS70_RS27100 (LTS70_27100)	6186103..6186522	-	420	WP_023367399.1	hypothetical protein	-
LTS70_RS27105 (LTS70_27105)	6186559..6187341	-	783	WP_036393899.1	VOC family protein	-
LTS70_RS27110 (LTS70_27110)	6187449..6187886	-	438	WP_023367403.1	SRPBCC family protein	Toxin
LTS70_RS27115 (LTS70_27115)	6187891..6188070	-	180	WP_023367405.1	antitoxin	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-141)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15888.48 Da
• Isoelectric Point: 8.5679

>T227828 WP_023367403.1 NZ_CP089216:c6187886-6187449 [Mycobacterium kansasii]
MAKLSGSIDVPLPPEEAWLHASNLSRYREWLTIHRVWRSKLPETLERGAVVESIVEVKGMPNRIRWTIVRYKPPEGMTLN
GDGVGGVKVKLMAKVAPKEEGSVVSFDVHLGGPALFGPIGMIVAAALRSDISESLQNFVEVFAQA

Nucleotide

• Length: 438 bp

>T227828 NZ_CP123047:c16333-15917 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6552.35 Da
• Isoelectric Point: 5.7603

>AT227828 WP_023367405.1 NZ_CP089216:c6188070-6187891 [Mycobacterium kansasii]
MGIMDKAKDLLSQNADKVETVIDKAGKFVDDKTQGKYSDTIHKVQEQAKKVVDTGDQQN

Nucleotide

• Length: 180 bp

>AT227828 NZ_CP123047:c16560-16330 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: X7ZGA3