Detailed information of TA system TA227827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): CapRel-ATcapRel/-
• Location: 6078311..6079215
• Replicon: chromosome
• Accession: NZ_CP089216
• Organism: Mycobacterium kansasii strain FDAARGOS_1615

Toxin (Protein)

• Gene name: capRel
• Uniprot ID: X7ZGG2
• Locus tag: LTS70_RS26610
• Protein ID: WP_023367202.1
• Coordinates: 6078562..6079215 (-)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: ATcapRel
• Uniprot ID: X7ZF14
• Locus tag: LTS70_RS26605
• Protein ID: WP_023367200.1
• Coordinates: 6078311..6078565 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTS70_RS26590 (LTS70_26590)	6074254..6075726	-	1473	WP_023367193.1	O-antigen ligase family protein	-
LTS70_RS26595 (LTS70_26595)	6075723..6077117	-	1395	WP_023367196.1	polysaccharide biosynthesis tyrosine autokinase	-
LTS70_RS26600 (LTS70_26600)	6077483..6078277	+	795	WP_036393567.1	FkbM family methyltransferase	-
LTS70_RS26605 (LTS70_26605)	6078311..6078565	-	255	WP_023367200.1	hypothetical protein	Antitoxin
LTS70_RS26610 (LTS70_26610)	6078562..6079215	-	654	WP_023367202.1	RelA/SpoT domain-containing protein	Toxin
LTS70_RS26620 (LTS70_26620)	6080472..6081617	-	1146	WP_036393847.1	hypothetical protein	-
LTS70_RS26625 (LTS70_26625)	6081805..6082458	-	654	WP_080674044.1	GNAT family protein	-
LTS70_RS26630 (LTS70_26630)	6082480..6083769	-	1290	WP_233013460.1	molybdopterin molybdotransferase MoeA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (53-150)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 24338.76 Da
• Isoelectric Point: 8.9815

>T227827 WP_023367202.1 NZ_CP089216:c6079215-6078562 [Mycobacterium kansasii]
VSTTIGPNEHGIEEIFQALDTLEAWRAAHARPLQAATMGLRSRVATMGCSQIEVSQRLERIPTIIDKLRREPGMNLGRMA
DIGGCRAVLRDVDEVRRVQSRYAGAAVTVRTRDYVEQPKSDGYRAVHVIVRYHGRLIEVQLRTQVQHEWAYTVESVTSRF
GLDIKAGGGPRPVHDWFVAVSEAMALEEYGEPVAPELLRRVDTLRAAAQPYLQGVRR

Nucleotide

• Length: 654 bp

>T227827 NZ_CP123046:c4635097-4634786 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9265.50 Da
• Isoelectric Point: 5.5094

>AT227827 WP_023367200.1 NZ_CP089216:c6078565-6078311 [Mycobacterium kansasii]
MTASLNHFLLVFDHSRAELVDLKNFGTDAKSAMAEYARLEREHMGKSDAIEIVLIGSDSLETVKVTHANYFDGTVAVSKY
FAGI

Nucleotide

• Length: 255 bp

>AT227827 NZ_CP123046:c4634785-4634495 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: X7ZGG2

Antitoxin

• AlphaFold DB: X7ZF14