Detailed information of TA system TA227826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 5321970..5322662
• Replicon: chromosome
• Accession: NZ_CP089216
• Organism: Mycobacterium kansasii strain FDAARGOS_1615

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7ZNI0
• Locus tag: LTS70_RS23375
• Protein ID: WP_023365904.1
• Coordinates: 5322240..5322662 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: X7ZK45
• Locus tag: LTS70_RS23370
• Protein ID: WP_036392951.1
• Coordinates: 5321970..5322233 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTS70_RS23335 (LTS70_23335)	5317042..5318544	-	1503	WP_023365894.1	trigger factor	-
LTS70_RS23350 (LTS70_23350)	5318905..5320089	+	1185	WP_023365896.1	serine hydrolase domain-containing protein	-
LTS70_RS23355 (LTS70_23355)	5320136..5320462	-	327	WP_133163539.1	hypothetical protein	-
LTS70_RS23360 (LTS70_23360)	5320550..5321356	-	807	WP_023365898.1	zinc finger domain-containing protein	-
LTS70_RS23365 (LTS70_23365)	5321390..5321869	-	480	WP_023365900.1	ribose-5-phosphate isomerase	-
LTS70_RS23370 (LTS70_23370)	5321970..5322233	+	264	WP_036392951.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LTS70_RS23375 (LTS70_23375)	5322240..5322662	+	423	WP_023365904.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTS70_RS23380 (LTS70_23380)	5322659..5323270	-	612	WP_023365906.1	Rv2466c family mycothiol-dependent reductase	-
LTS70_RS23385 (LTS70_23385)	5323411..5326014	+	2604	WP_036392948.1	aminopeptidase N	-
LTS70_RS23390 (LTS70_23390)	5326082..5326549	-	468	WP_036392579.1	DUF5130 domain-containing protein	-
LTS70_RS23395 (LTS70_23395)	5326536..5326763	-	228	WP_023365912.1	hypothetical protein	-
LTS70_RS23400 (LTS70_23400)	5326826..5327500	-	675	WP_036392577.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-83)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15092.17 Da
• Isoelectric Point: 5.0719

>T227826 WP_023365904.1 NZ_CP089216:5322240-5322662 [Mycobacterium kansasii]
VILDSSAFVALIQGEAPYTEQIAAALTGDRSPVMSTANAAECLIVLTSRHGFIARTVFDRLRFEINLGFQPFNFELAMAA
HLAYLRYGRGRHPAGLNCGDTMAYATAKLAHEPLIAIGNDFAQTDLESDGVIGYWPSPCG

Nucleotide

• Length: 423 bp

>T227826 NZ_CP123046:c4346161-4345988 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9890.16 Da
• Isoelectric Point: 5.6277

>AT227826 WP_036392951.1 NZ_CP089216:5321970-5322233 [Mycobacterium kansasii]
VAFNVKDEEVIRLADELAARLHHPSRIDAIRYALRAQIEITQSRTANRADELLDVLRTEIWPLLHDRSPITKSEREQALG
YDAATGV

Nucleotide

• Length: 264 bp

>AT227826 NZ_CP123046:c4346485-4346189 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: X7ZNI0

Antitoxin

• AlphaFold DB: X7ZK45