Detailed information of TA system TA227825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 5178549..5179126
• Replicon: chromosome
• Accession: NZ_CP089216
• Organism: Mycobacterium kansasii strain FDAARGOS_1615

Toxin (Protein)

• Gene name: doc
• Uniprot ID: X7ZN33
• Locus tag: LTS70_RS22765
• Protein ID: WP_023365653.1
• Coordinates: 5178743..5179126 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: X7ZMP8
• Locus tag: LTS70_RS22760
• Protein ID: WP_023365651.1
• Coordinates: 5178549..5178746 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTS70_RS22735 (LTS70_22735)	5173693..5174229	-	537	WP_023365641.1	rRNA maturation RNase YbeY	-
LTS70_RS22740 (LTS70_22740)	5174279..5175328	-	1050	WP_036392639.1	PhoH family protein	-
LTS70_RS22745 (LTS70_22745)	5175475..5176920	-	1446	WP_133163534.1	cytochrome P450	-
LTS70_RS22750 (LTS70_22750)	5177003..5177794	-	792	WP_023365647.1	class I SAM-dependent methyltransferase	-
LTS70_RS22755 (LTS70_22755)	5177864..5178433	-	570	WP_023365649.1	carboxymethylenebutenolidase	-
LTS70_RS22760 (LTS70_22760)	5178549..5178746	+	198	WP_023365651.1	hypothetical protein	Antitoxin
LTS70_RS22765 (LTS70_22765)	5178743..5179126	+	384	WP_023365653.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LTS70_RS22770 (LTS70_22770)	5179149..5179895	-	747	WP_023365655.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
LTS70_RS22775 (LTS70_22775)	5179914..5181062	-	1149	WP_023365657.1	molecular chaperone DnaJ	-
LTS70_RS22780 (LTS70_22780)	5181122..5182153	-	1032	WP_023365659.1	heat-inducible transcriptional repressor HrcA	-
LTS70_RS22785 (LTS70_22785)	5182369..5182683	+	315	WP_023365661.1	type II toxin-antitoxin system VapB family antitoxin	-
LTS70_RS22790 (LTS70_22790)	5182841..5183190	+	350	Protein_4513	hypothetical protein	-
LTS70_RS22795 (LTS70_22795)	5183222..5183801	+	580	Protein_4514	sigma factor-like helix-turn-helix DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (19-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13726.39 Da
• Isoelectric Point: 3.9949

>T227825 WP_023365653.1 NZ_CP089216:5178743-5179126 [Mycobacterium kansasii]
VTEFFDRDDVLTAGALAVGAVLEVGDFGLLDAAVARPQATVYGVDAYPDLYTKAAALLQSLARNRALVDGNKRTAWASAW
TFLYINGIELVAGFDVDTAERFMYDVAERGELTVDDIAETLRQFASA

Nucleotide

• Length: 384 bp

>T227825 NZ_CP123046:c4249842-4249492 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6940.89 Da
• Isoelectric Point: 11.0918

>AT227825 WP_023365651.1 NZ_CP089216:5178549-5178746 [Mycobacterium kansasii]
VPSLNIGFTDEELVAVRDAAAGENLSLRAFVHRAAVVAASDRKRRVAEAAALVAQRSAELNRRLA

Nucleotide

• Length: 198 bp

>AT227825 NZ_CP123046:c4250087-4249836 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCTTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: X7ZN33

Antitoxin

• AlphaFold DB: X7ZMP8